Occurrence and prevalence of antibiotic resistance in landfill leachate

doi:10.1007/s11356-015-4514-7

	Occurrence and prevalence of antibiotic resistance in landfill leachate
	Wang, Yangqing; Tang, Wei; Qiao, Jing; Song, Liyan
刊名	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
	2015-08-01
卷号	22 期号:16 页码:12525-12533
关键词	Landfill Landfill leachate Antibiotic resistance (AR) Antibiotic-resistant bacteria (ARB) Antibiotic-resistant genes (ARGs)
ISSN号	0944-1344
DOI	10.1007/s11356-015-4514-7
通讯作者	Song, LY (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Res Ctr Environm Microbiol & Ecol, Chongqing 401122, Peoples R China.
英文摘要	Antibiotic resistance (AR) is extensively present in various environments, posing emerging threat to public and environmental health. Landfill receives unused and unwanted antibiotics through household waste and AR within waste (e.g., activated sludge and illegal clinical waste) and is supposed to serve as an important AR reservoir. In this study, we used culture-dependent methods and quantitative molecular techniques to detect and quantify antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in 12 landfill leachate samples from six geographic different landfills, China. Five tested ARGs (tetO, tetW, bla(TEM), sulI, and sulII) and seven kinds of antibiotic-resistant heterotrophic ARB were extensively detected in all samples, demonstrating their occurrence in landfill. The detected high ratio (10(-2) to 10(-5)) of ARGs to 16S ribosomal RNA (rRNA) gene copies implied that ARGs are prevalent in landfill. Correlation analysis showed that ARGs (tetO, tetW, sulI, and sulII) significantly correlated to ambient bacterial 16S rRNA gene copies, suggesting that the abundance of bacteria in landfill leachate may play an important role in the horizontal spread of ARGs.
资助项目	Chinese Academy of Science, China[KZCX2-XB3-14]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000358579700052
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/1811]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Song, Liyan
作者单位	Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Res Ctr Environm Microbiol & Ecol, Chongqing 401122, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yangqing,Tang, Wei,Qiao, Jing,et al. Occurrence and prevalence of antibiotic resistance in landfill leachate[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2015,22(16):12525-12533.
APA	Wang, Yangqing,Tang, Wei,Qiao, Jing,&Song, Liyan.(2015).Occurrence and prevalence of antibiotic resistance in landfill leachate.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,22(16),12525-12533.
MLA	Wang, Yangqing,et al."Occurrence and prevalence of antibiotic resistance in landfill leachate".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 22.16(2015):12525-12533.