Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies

doi:10.1016/j.snb.2017.08.205

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 太赫兹技术研究中心

	Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies
	Yun, Wen 1; Wu, Hong 1; Liu, Xingyan 1; Zhong, Haixia 1; Fu, Min 1; Yang, Lizhu 2; Huang, Yu3
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-02-01
卷号	255 页码:1920-1926
关键词	DNAzyme Gold nanoparticles Fluorescent Colorimetric Dual amplification
ISSN号	0925-4005
DOI	10.1016/j.snb.2017.08.205
通讯作者	Yun, W (reprint author), Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China. ; Yang, LZ (reprint author), Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China.
英文摘要	A novel fluorescent and colorimetric strategy with enzyme-free dual amplification was developed for ultra-sensitively detection of uranyl in river waters samples. The enzyme-strand recycling and hairpin catalytic assembly (HCA) reaction were used as dual enzyme-free amplification strategies. Three hairpins labeled with fluorophore can absorb on the surface of gold nanoparticles (AuNPs) to prevent them from aggregation in salt solution and quench the fluorescent signal simultaneously. In the presence of UO22+, the HCA reaction between hairpins was induced to form rigid DNA triangles, resulting in the aggregation of AuNPs with a red to blue color change and a "turn-on" fluorescent signal. The limit of detection was as low as 0.1 pM. Importantly, this strategy provided colorimetric and fluorescent dual signals for lower false-positives and higher anti-interference ability. It was successfully used for detection of uranyl in real waters samples. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[31300819] ; Project of Wenzhou Science &Technology Bureau[S20150012] ; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences[201712] ; Open Research Fund[CQCM-2016-05] ; Science and Technology Research Program of Chongqing Municipal Education Commission[KJ1706156] ; Startup Foundation of Chongqing Technology and Business University[1756001] ; funds of China Academy of Engineering Physics[TCSQ2016203] ; Open Research Fund Program of Key Laboratory of Reservoir Aquatic Environment of CAS
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000414319900089
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/80]
专题	太赫兹技术研究中心
通讯作者	Yun, Wen; Yang, Lizhu
作者单位	1.Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China 2.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Yun, Wen,Wu, Hong,Liu, Xingyan,et al. Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:1920-1926.
APA	Yun, Wen.,Wu, Hong.,Liu, Xingyan.,Zhong, Haixia.,Fu, Min.,...&Huang, Yu.(2018).Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies.SENSORS AND ACTUATORS B-CHEMICAL,255,1920-1926.
MLA	Yun, Wen,et al."Ultra-sensitive fluorescent and colorimetric detection of UO22+ based on dual enzyme-free amplification strategies".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):1920-1926.