An Integrated Proteomic and Metabolomic Study on the Chronic Effects of Mercury in Suaeda salsa under an Environmentally Relevant Salinity

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	An Integrated Proteomic and Metabolomic Study on the Chronic Effects of Mercury in Suaeda salsa under an Environmentally Relevant Salinity
	Liu, Xiaoli 1,2,3; Wu, Huifeng1,2 ; Ji, Chenglong1,2,3 ; Wei, Lei 1,2,3; Zhao, Jianmin1,2 ; Yu, Junbao1,2
刊名	PLOS ONE
	2013-05-16
卷号	8 期号:5 页码:e64041
关键词	Laser-desorption/ionization-time Protein Disulfide-isomerase Oxidative Stress Aspartate-aminotransferase Saccharomyces-cerevisiae Gene-expression Arabidopsis Tolerance Responses Identification
ISSN号	1932-6203
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China. hfwu@yic.ac.cn
产权排序	[Liu, Xiaoli; Wu, Huifeng; Ji, Chenglong; Wei, Lei; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China; [Liu, Xiaoli; Wu, Huifeng; Ji, Chenglong; Wei, Lei; Zhao, Jianmin; Yu, Junbao] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China; [Liu, Xiaoli; Ji, Chenglong; Wei, Lei] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
文献子类	Article
英文摘要	As an environmental contaminant, mercury is of great concern due to its high risk to environmental and human health. The halophyte Suaeda salsa is the dominant plant in the intertidal zones of the Yellow River Delta (YRD) where has been contaminated by mercury in some places. This study aimed at evaluating the chronic effects of mercury (Hg2+, 20 mu g L-1) and the influence of an environmentally relevant salinity (NaCl, 500 mM) on mercury-induced effects in S. salsa. A total of 43 protein spots with significant changes were identified in response to Hg2+, salinity and combined Hg2+ and salinity. These proteins can be categorized into diverse functional classes, related to metabolic processes, photosynthesis, stress response, protein fate, energy metabolism, signaling pathways and immunosuppression. Metabolic responses demonstrated that Hg2+ could disturb protein and energy metabolisms in S. salsa co-exposed with or without salinity. In addition, both antagonistic and synergistic effects between Hg2+ and salinity were confirmed by differential levels of proteins (magnesium-chelatase and ribulose-l,5-bisphosphate carboxylase/oxygenase) and metabolites (valine, malonate, asparagine, glycine, fructose and glucose) in S. salsa. These findings suggest that a combination of proteomics and metabolomics can provide insightful information of environmental contaminant-induced effects in plants at molecular levels.; As an environmental contaminant, mercury is of great concern due to its high risk to environmental and human health. The halophyte Suaeda salsa is the dominant plant in the intertidal zones of the Yellow River Delta (YRD) where has been contaminated by mercury in some places. This study aimed at evaluating the chronic effects of mercury (Hg2+, 20 mu g L-1) and the influence of an environmentally relevant salinity (NaCl, 500 mM) on mercury-induced effects in S. salsa. A total of 43 protein spots with significant changes were identified in response to Hg2+, salinity and combined Hg2+ and salinity. These proteins can be categorized into diverse functional classes, related to metabolic processes, photosynthesis, stress response, protein fate, energy metabolism, signaling pathways and immunosuppression. Metabolic responses demonstrated that Hg2+ could disturb protein and energy metabolisms in S. salsa co-exposed with or without salinity. In addition, both antagonistic and synergistic effects between Hg2+ and salinity were confirmed by differential levels of proteins (magnesium-chelatase and ribulose-l,5-bisphosphate carboxylase/oxygenase) and metabolites (valine, malonate, asparagine, glycine, fructose and glucose) in S. salsa. These findings suggest that a combination of proteomics and metabolomics can provide insightful information of environmental contaminant-induced effects in plants at molecular levels.
学科主题	Multidisciplinary Sciences
URL标识	查看原文
WOS关键词	LASER-DESORPTION/IONIZATION-TIME ; PROTEIN DISULFIDE-ISOMERASE ; OXIDATIVE STRESS ; ASPARTATE-AMINOTRANSFERASE ; SACCHAROMYCES-CEREVISIAE ; GENE-EXPRESSION ; ARABIDOPSIS ; TOLERANCE ; RESPONSES ; IDENTIFICATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000319081900068
资助机构	National Science & Technology Pillar Program in "12th Five Year'' Period [2011BAC02B01]; 100 Talents Program of the Chinese Academy of Sciences
公开日期	2013-08-15
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6595]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_滨海湿地实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaoli,Wu, Huifeng,Ji, Chenglong,et al. An Integrated Proteomic and Metabolomic Study on the Chronic Effects of Mercury in Suaeda salsa under an Environmentally Relevant Salinity[J]. PLOS ONE,2013,8(5):e64041.
APA	Liu, Xiaoli,Wu, Huifeng,Ji, Chenglong,Wei, Lei,Zhao, Jianmin,&Yu, Junbao.(2013).An Integrated Proteomic and Metabolomic Study on the Chronic Effects of Mercury in Suaeda salsa under an Environmentally Relevant Salinity.PLOS ONE,8(5),e64041.
MLA	Liu, Xiaoli,et al."An Integrated Proteomic and Metabolomic Study on the Chronic Effects of Mercury in Suaeda salsa under an Environmentally Relevant Salinity".PLOS ONE 8.5(2013):e64041.