Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte) | |
Chen, Peng1,4; Shao, HongBo1,2; Xu, Di3 | |
刊名 | AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH |
2011-09-16 | |
卷号 | 5期号:18页码:2590-2595 |
关键词 | Gracilaria Lemaneiformis Suppression Subtractive Hybridization (Ssh) Sex Determination Gametophyte |
ISSN号 | 1996-0808 |
通讯作者 | Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China. shaohongbochu@126.com |
产权排序 | [Chen, Peng; Shao, HongBo] Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China; [Shao, HongBo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Xu, Di] Ocean Univ China, Coll Marine Life Sci, Qingdao 266002, Peoples R China; [Chen, Peng] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
文献子类 | Article |
英文摘要 | In this study, forward and reverse suppression subtractive hybridization (SSH) libraries were constructed between female and male gametophyte of Gracilaria lemaneiformis to isolate genes differentially expressed between gametophytes. Dot-blots were performed to screen 384 colonies randomly selected from each subtracted libraries. Partial gene (designated as GMF-01) which is female gametophyte-specific was selected to isolate its cDNA full length sequences and to be characterized. Then we got its cDNA full length sequences using SMART-RACE technic. Sequence analysis showed that the open reading frame (ORF) of GMF-01 is 1002 bp long with a GC content of 47.7%, encoding 333 amino acids. GMF-01 does not have significant match in the databases when examined on NCBI website. Amino acid sequence analysis showed that the theoretical pI of this translated protein is 7.92, while the instability index is computed to be 43.61, which has classified the protein as unstable. Results of prediction showed 45.05% of its secondary structure has random coil and sub-cellular location prediction indicated that it is probably a extracellular protein.; In this study, forward and reverse suppression subtractive hybridization (SSH) libraries were constructed between female and male gametophyte of Gracilaria lemaneiformis to isolate genes differentially expressed between gametophytes. Dot-blots were performed to screen 384 colonies randomly selected from each subtracted libraries. Partial gene (designated as GMF-01) which is female gametophyte-specific was selected to isolate its cDNA full length sequences and to be characterized. Then we got its cDNA full length sequences using SMART-RACE technic. Sequence analysis showed that the open reading frame (ORF) of GMF-01 is 1002 bp long with a GC content of 47.7%, encoding 333 amino acids. GMF-01 does not have significant match in the databases when examined on NCBI website. Amino acid sequence analysis showed that the theoretical pI of this translated protein is 7.92, while the instability index is computed to be 43.61, which has classified the protein as unstable. Results of prediction showed 45.05% of its secondary structure has random coil and sub-cellular location prediction indicated that it is probably a extracellular protein. |
学科主题 | Microbiology |
WOS关键词 | SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; RED ALGA ; IDENTIFICATION ; DIFFERENTIATION ; EXPRESSION ; LIBRARIES |
WOS研究方向 | Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000298919300006 |
资助机构 | National Scientific Foundations of China[40606034] |
公开日期 | 2012-03-06 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/5307] |
专题 | 烟台海岸带研究所_滨海湿地实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Environm Proc, CAS Shandong Prov, Yantai 264003, Peoples R China 2.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266002, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Peng,Shao, HongBo,Xu, Di. Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(18):2590-2595. |
APA | Chen, Peng,Shao, HongBo,&Xu, Di.(2011).Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte).AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(18),2590-2595. |
MLA | Chen, Peng,et al."Cloning and characterization of a female gametophyte-specific gene in Gracilaria lemaneiformis (Gracilariales, Rhodophyte)".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.18(2011):2590-2595. |
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