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	Applications of molecular biology and biotechnology in oil field microbial biodiversity research
	Wen, Zhuang1,2,4; Hong-Bo, Shao1,3
刊名	AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
	2011-09-30
卷号	5期号:20页码:3103-3112
关键词	Biotechnology Molecular Biology Techniques Oil Field 16s Rrna Microbial Biodiversity Prospect
ISSN号	1996-0808
通讯作者	Hong-Bo, S (reprint author), Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
产权排序	[Wen, Zhuang; Hong-Bo, Shao] Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wen, Zhuang] Zaozhuang Vocat Coll, Zaozhuang 277800, Peoples R China; [Hong-Bo, Shao] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; [Wen, Zhuang] Chinese Acad Sci CAS, Grad Univ, Beijing 100049, Peoples R China
文献子类	Review
英文摘要	In recent years, with more and more attention given to the study of microorganisms in oil fields, molecular biology and biotechnology methodology has been applied prosperously. This article described the most commonly-used molecular biology techniques and related biotechnological advances in oil fields research at present, which are based mainly on 16s rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc. The article also points out their advantages and disadvantages, respectively and introduces some of the micro-organisms, which are of largest concerns in the research.; In recent years, with more and more attention given to the study of microorganisms in oil fields, molecular biology and biotechnology methodology has been applied prosperously. This article described the most commonly-used molecular biology techniques and related biotechnological advances in oil fields research at present, which are based mainly on 16s rRNA, such as 16S rRNA sequencing, denaturant gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc. The article also points out their advantages and disadvantages, respectively and introduces some of the micro-organisms, which are of largest concerns in the research.
学科主题	Microbiology
WOS关键词	GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; SULFATE-REDUCING BACTERIA ; ACTIVATED-SLUDGE ; H2S PRODUCTION ; DNA FRAGMENTS ; PCR ; DIVERSITY ; POPULATIONS ; COMMUNITY
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000298925600002
资助机构	Chinese Academy of Sciences (CAS)[KZCX2-YW-JC203, 2009Y2B211]; CAS/SAFEA
公开日期	2012-03-06
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5303]
专题	烟台海岸带研究所_滨海湿地实验室
作者单位	1.Chinese Acad Sci CAS, CAS Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Zaozhuang Vocat Coll, Zaozhuang 277800, Peoples R China 3.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China 4.Chinese Acad Sci CAS, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wen, Zhuang,Hong-Bo, Shao. Applications of molecular biology and biotechnology in oil field microbial biodiversity research[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(20):3103-3112.
APA	Wen, Zhuang,&Hong-Bo, Shao.(2011).Applications of molecular biology and biotechnology in oil field microbial biodiversity research.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(20),3103-3112.
MLA	Wen, Zhuang,et al."Applications of molecular biology and biotechnology in oil field microbial biodiversity research".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.20(2011):3103-3112.

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