Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction
	Zhang, Weiwei 1,2; Chen, Lingxin1,2 ; Liu, Dongyan1,2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2012-02-01
卷号	93 期号:3 页码:1305-1314
关键词	Pseudomonas Putida Marine Environment Mer Operon Bioremediation Of Hgcl2 Contamination
ISSN号	0175-7598
产权排序	(1)Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China; (2)Key Laboratory of Coastal Zone Environmental Processes, Chinese Academy of Sciences; Shandong Provin, Chinese Academy of Sciences, Yantai, China
文献子类	Article
英文摘要	The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resist © 2011 Springer-Verlag.; The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 mu M HgCl2. SP1 was also highly resistant to other metals, including CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, and ZnSO4, and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl2 and the removal of HgCl2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg-0 vapor, was around 5.0. LD50 of P. putida SP1 to flounder and turbot was 1.5x 10(9) CFU. Biofilm developed by P. putida SP1 was 1- to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl2 contamination over a broad range of pH.
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WOS关键词	BACTERIA HIGHLY RESISTANT ; ESCHERICHIA-COLI ; MOLECULAR APPLICATION ; WASTE-WATER ; MER OPERON ; TRANSPOSONS ; BIOREMEDIATION ; TEMPERATURE ; SEQUENCES ; VIRULENCE
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000300282500038
公开日期	2011-07-25
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/4941]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weiwei,Chen, Lingxin,Liu, Dongyan. Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(3):1305-1314.
APA	Zhang, Weiwei,Chen, Lingxin,&Liu, Dongyan.(2012).Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(3),1305-1314.
MLA	Zhang, Weiwei,et al."Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.3(2012):1305-1314.