Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok

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	Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok
	Li, Zheng 1; Zhang, Songyan 1; Gao, Jiuxiang 1; Guang, Huijuan 1; Tian, Yuanyong 2; Zhao, Zongmao 2,4; Wang, Yipeng3 ; Yu, Haining 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013
卷号	34 期号:1 页码:1-7
关键词	Lenok Cathelicidin Molecular Cloning Transcript Levels Functional Characterization 3d Structure Modeling
ISSN号	1050-4648
通讯作者	Wang, YP (reprint author), Linggong Rd 2, Dalian, Liaoning, Peoples R China. wyp010@163.com ; joannyu@live.cn
产权排序	[Li, Zheng; Zhang, Songyan; Gao, Jiuxiang; Guang, Huijuan; Yu, Haining] Dalian Univ Technol, Sch Life Sci & Biotechnol, Inst Marine Biol Technol, Dalian 116024, Liaoning, Peoples R China; [Tian, Yuanyong; Zhao, Zongmao; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Hebei, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China; [Zhao, Zongmao] Hebei Med Univ, Hosp 2, Dept Neurosurg, Shijiazhuang 050000, Peoples R China
文献子类	Article
英文摘要	Thick-lipped lenok, Brachymystax lenok is one of the ancient fish species in China and northeast Asia countries. Due to the overfishing, the population of lenok has been declined significantly. Cathelicidins are innate immune effectors that possess both bactericidal activities and immunomodulatory functions. This report identifies and characterizes the salmonoid cathelicidin (CATH_BRALE) from this ancient fish. It consists of open reading frame (ORF) of 886 bp encoding the putative peptide of 199 amino acids. Sequence alignment with other representative salmonid cathelicidins displayed two distinctive features of current lenok cathelicidin: high level of arginine, resulting in high positive charge and glycine residues, which is significantly different from most acknowledged types of cathelicidins; and the six-amino-acid tandem repeated sequence of RPGGGS detected in a variable number of copies among fish cathelicidins, suggesting the existence of a genetically unstable region similar to that found in some mammalian cathelicidins. Expression of CATH_BRALE is predominantly found in gill, with lower levels in the gastrointestinal tract and spleen. The homology modeled structure of CATH_BRALE exhibits structural features of antiparallel beta-sheets flanked by alpha-helices that are representative of small cationic cathelicidin family peptides. CATH_BRALE possesses much stronger antimicrobial activity against gram-negative bacteria than that of the human ortholog, LL-37. The growth of two typical fish bacterial pathogens, gram-negative bacterium of Aeromonas salmonicida and Aeromonas hydrophila was substantially inhibited by synthetic CATH_BRALE, with both MICs as low as 9.38 mu M. (c) 2012 Elsevier Ltd. All rights reserved.; Thick-lipped lenok, Brachymystax lenok is one of the ancient fish species in China and northeast Asia countries. Due to the overfishing, the population of lenok has been declined significantly. Cathelicidins are innate immune effectors that possess both bactericidal activities and immunomodulatory functions. This report identifies and characterizes the salmonoid cathelicidin (CATH_BRALE) from this ancient fish. It consists of open reading frame (ORF) of 886 bp encoding the putative peptide of 199 amino acids. Sequence alignment with other representative salmonid cathelicidins displayed two distinctive features of current lenok cathelicidin: high level of arginine, resulting in high positive charge and glycine residues, which is significantly different from most acknowledged types of cathelicidins; and the six-amino-acid tandem repeated sequence of RPGGGS detected in a variable number of copies among fish cathelicidins, suggesting the existence of a genetically unstable region similar to that found in some mammalian cathelicidins. Expression of CATH_BRALE is predominantly found in gill, with lower levels in the gastrointestinal tract and spleen. The homology modeled structure of CATH_BRALE exhibits structural features of antiparallel beta-sheets flanked by alpha-helices that are representative of small cationic cathelicidin family peptides. CATH_BRALE possesses much stronger antimicrobial activity against gram-negative bacteria than that of the human ortholog, LL-37. The growth of two typical fish bacterial pathogens, gram-negative bacterium of Aeromonas salmonicida and Aeromonas hydrophila was substantially inhibited by synthetic CATH_BRALE, with both MICs as low as 9.38 mu M. (c) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
WOS关键词	GLYCINE-RICH PROTEINS ; ANTIMICROBIAL PEPTIDES ; ONCORHYNCHUS-MYKISS ; CATHELICIDIN GENE ; INNATE IMMUNITY ; RAINBOW-TROUT ; FAMILY ; SEQUENCE
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000314743600001
资助机构	Chinese National Natural Science Foundation [30900240, 41076098]
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6459]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Inst Marine Biol Technol, Dalian 116024, Liaoning, Peoples R China 2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Hebei, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Biol Resources Lab, Yantai 264003, Shandong, Peoples R China 4.Hebei Med Univ, Hosp 2, Dept Neurosurg, Shijiazhuang 050000, Peoples R China
推荐引用方式 GB/T 7714	Li, Zheng,Zhang, Songyan,Gao, Jiuxiang,et al. Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):1-7.
APA	Li, Zheng.,Zhang, Songyan.,Gao, Jiuxiang.,Guang, Huijuan.,Tian, Yuanyong.,...&Yu, Haining.(2013).Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok.FISH & SHELLFISH IMMUNOLOGY,34(1),1-7.
MLA	Li, Zheng,et al."Structural and functional characterization of CATH_BRALE, the defense molecule in the ancient salmonoid, Brachymystax lenok".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):1-7.