Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop

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	Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop
	Jin, Shanzhao 1,2; Liu, Ling 1; Liu, Zhaopu 1,2; Long, Xiaohua 1,2; Shao, Hongbo 1,2,4; Chen, Jiayao 3
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2013-05-01
卷号	43 页码:556-561
关键词	Jerusalem Artichoke Industrial Crop Storage Marine Bacteria Antifungal Pseudomonas Spp.
ISSN号	0926-6690
通讯作者	Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn ; shaohongbochu@126.com
产权排序	[Jin, Shanzhao; Liu, Ling; Liu, Zhaopu; Long, Xiaohua; Shao, Hongbo] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Jin, Shanzhao; Liu, Zhaopu; Long, Xiaohua; Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Chen, Jiayao] Beijing Univ Technol, Beijing 100022, Peoples R China; [Shao, Hongbo] QUST, Inst Life Sci, Qingdao 266042, Peoples R China
文献子类	Article
英文摘要	The aim of this study was the isolation, characterization, and selection of Pseudomonas spp. with antagonistic activity against Jerusalem artichoke tuber rot disease during storage. Ten pathogenic fungi were isolated from rotting tubers and classified in seven genera based on morphology and the part sequences in the ITS analysis. The marine Pseudomonas spp. strain JK2, isolated from mud in South China Sea, exhibited high antifungal activity against Aspergillus tamari (M10), Fusarium solani (M9), and Aspergillus fumigatus (M2). JK2 was identified as Pseudomonas spp. based on morphological, physiological and biochemical methods as well as 16S rDNA analysis. Seven days after inoculation the radii of the inhibition zone (mm) of M2, MO and M10 were 10.5 +/- 1.2, 6.5 +/- 0.15, and 13.0 +/- 0.75, respectively, compared to the sterilized medium as control. Transmission electron microscopy showed that, compared with the normal hyphae, the fungal hyphae treated with strain JK2 had injury in the cell wall. Thus, JK2 had a significant fungicidal effect on the three pathogenic fungi. To our best knowledge, this is the first report of biocontrol agent for storage disease of Jerusalem artichoke. 2012 Elsevier B.V. All rights reserved.; The aim of this study was the isolation, characterization, and selection of Pseudomonas spp. with antagonistic activity against Jerusalem artichoke tuber rot disease during storage. Ten pathogenic fungi were isolated from rotting tubers and classified in seven genera based on morphology and the part sequences in the ITS analysis. The marine Pseudomonas spp. strain JK2, isolated from mud in South China Sea, exhibited high antifungal activity against Aspergillus tamari (M10), Fusarium solani (M9), and Aspergillus fumigatus (M2). JK2 was identified as Pseudomonas spp. based on morphological, physiological and biochemical methods as well as 16S rDNA analysis. Seven days after inoculation the radii of the inhibition zone (mm) of M2, MO and M10 were 10.5 +/- 1.2, 6.5 +/- 0.15, and 13.0 +/- 0.75, respectively, compared to the sterilized medium as control. Transmission electron microscopy showed that, compared with the normal hyphae, the fungal hyphae treated with strain JK2 had injury in the cell wall. Thus, JK2 had a significant fungicidal effect on the three pathogenic fungi. To our best knowledge, this is the first report of biocontrol agent for storage disease of Jerusalem artichoke. 2012 Elsevier B.V. All rights reserved.
学科主题	Agricultural Engineering ; Agronomy
URL标识	查看原文
WOS关键词	HELIANTHUS-TUBEROSUS ; BIOACTIVE COMPOUNDS ; SEAWATER STRESS ; BACTERIA ; L. ; ETHANOL ; YEAST ; RHIZOSPHERE ; BIOCONTROL ; PATHOGENS
WOS研究方向	Agriculture
语种	英语
WOS记录号	WOS:000311865000083
资助机构	Ministry of Science and Technology of China [2011BAD13B09]; Ministry of Science and Technology of Jiangsu Province [BE2010305, BE2011368]; Fundamental Research Funds for Central Universities [Y0201100249]; New Teachers' Fund for Doctor Stations, Ministry of Education [20100097120016]; Project of a Special Fund for Public Welfare Industrial (Agriculture) Research of China [200903001-5]; National Natural Science Foundation of China [41171216]; One Hundred-Talent Plan of CAS; CAS/SAFEA International Partnership Program for Creative Research Teams; National Key Projects of Scientific and Technical Support Programs
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6417]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Beijing Univ Technol, Beijing 100022, Peoples R China 4.QUST, Inst Life Sci, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Jin, Shanzhao,Liu, Ling,Liu, Zhaopu,et al. Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop[J]. INDUSTRIAL CROPS AND PRODUCTS,2013,43:556-561.
APA	Jin, Shanzhao,Liu, Ling,Liu, Zhaopu,Long, Xiaohua,Shao, Hongbo,&Chen, Jiayao.(2013).Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop.INDUSTRIAL CROPS AND PRODUCTS,43,556-561.
MLA	Jin, Shanzhao,et al."Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop".INDUSTRIAL CROPS AND PRODUCTS 43(2013):556-561.