Method of mRNA display for selecting peptides that bind with target enzyme RNA

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Method of mRNA display for selecting peptides that bind with target enzyme RNA
	Yan,Song ; Wang,Zheng ; Yang,Shao-Li ; Zhang,Yi ; Lin,Xiu-Kun
	2011
会议日期	2011-05-13
会议地点	Wuhan, China
关键词	Peptides Bioinformatics Medicine Molecular Biology Rna
通讯作者	Yan, S. (yansong209@yahoo.com.cn)
英文摘要	In vitro display techniques are useful tools for selecting novel ligands that bind to target biomolecules. In this paper, mRNA display method was used to select peptides that bind thymidylate synthase RNA with high affinity from a peptide library. We provide a detailed analysis of the critical steps of an mRNA-display-based selection, including the generation of an mRNA-displayed library, formation of RNA-peptide fusions, and selection of RNA affinity peptides. After optimization of the experimental steps, the efficiency of RNA-linker purification and the reverse transcription was improved, and the unspecified binding peptides were removed. Our study could facilitate the use of mRNA display in the field of biotechnology, medicine and proteomics. © 2011 IEEE.
产权排序	(1)Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; (2)School of Environmental Science and Engineering, Dalian Jiaotong University, Dalian 116028, China; (3)Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, 264003, China
会议录	8th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
URL标识	查看原文
内容类型	会议论文
源URL	[http://ir.yic.ac.cn/handle/133337/4935]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	Yan,Song,Wang,Zheng,Yang,Shao-Li,et al. Method of mRNA display for selecting peptides that bind with target enzyme RNA[C]. 见:. Wuhan, China. 2011-05-13.