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	Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene
	Yan, Mingyan1,2; Li, Bafang1; Zhao, Xue1
刊名	FOOD CHEMISTRY
	2010-10-15
卷号	122期号:4页码:1333-1337
关键词	Collagen Pyrene Aggregation Walleye Pollock Fish Skin
ISSN号	0308-8146
通讯作者	Li, BF, Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
产权排序	[Yan, Mingyan; Li, Bafang; Zhao, Xue] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China; [Yan, Mingyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
文献子类	Article
英文摘要	The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.; The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
URL标识	查看原文
WOS关键词	CRITICAL MICELLE CONCENTRATION ; AQUEOUS-SOLUTION ; SURFACTANT
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
WOS记录号	WOS:000278582400062
资助机构	National Natural Science Foundation of China [30871943]; High Technology Research and Development Programme of China [2006AA09Z438]
公开日期	2011-07-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3752]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yan, Mingyan,Li, Bafang,Zhao, Xue. Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene[J]. FOOD CHEMISTRY,2010,122(4):1333-1337.
APA	Yan, Mingyan,Li, Bafang,&Zhao, Xue.(2010).Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene.FOOD CHEMISTRY,122(4),1333-1337.
MLA	Yan, Mingyan,et al."Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene".FOOD CHEMISTRY 122.4(2010):1333-1337.

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