Introduction to Molecular Analysis of Ectomycorrhizal Communities

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	Introduction to Molecular Analysis of Ectomycorrhizal Communities
	MARTIN Kendall J.
刊名	Soil Science Society of America journal
	2007
卷号	71 期号:2 页码: 601-610
关键词	Soil Biology Soil Science Mycology Microbiology Ecology Molecular Biology Biochemical Compound Nucleic Acid Eukaryote Symbiosis Symbiont Mycorrhiza Mycoflora Molecular Methods Molecular Analysis Polymerase Chain Reaction Ribosomal Rna Gene Ribosomal Dna Spacer Dna Dna Fungi Root Apex Analysis Method Microbial Community Ectomycorrhiza Review
ISSN号	0361-5995
通讯作者	MARTIN Kendall J.
英文摘要	A number of methods are available for those researchers considering the addition of molecular analyses of ectomycorrhizal (EcM) fungi to their research projects and weighing the various approaches they might take. Analyzing natural EcM fungal communities has traditionally been a highly skilled, time-consuming process relying heavily on exacting morphological characterization of EcM root tips. Increasingly powerful molecular methods for analyzing EcM communities make this area of research available to a much wider range of researchers. Ecologists can gain from the body of work characterizing EcM while avoiding the requirement for exceptional expertise by carefully combining elements of traditional methods with the more recent molecular approaches. A cursory morphological analysis can yield a traditional quantification of EcM fungi based on tip numbers, a unit with functional and historical significance. Ectomycorrhizal root DNA extracts may then be analyzed with molecular methods widely used for characterizing microbiota. These range from methods applicable only to the simple mixes resulting from careful morphotyping, to community-oriented methods that identify many types in mixed samples as well as provide an estimate of their relative abundances. Extramatrical hyphae in bulk soil can also be more effectively studied, extending characterization of EcM fungal communities beyond the rhizoplane. The trend toward techniques permitting larger sample sets without prohibitive labor and time requirements will also permit us to more frequently address the issues of spatial and temporal variability and better characterize the roles of EcM fungi at multiple scales.
公开日期	2010-12-27
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3012]
专题	烟台海岸带研究所_科研共享资源
推荐引用方式 GB/T 7714	MARTIN Kendall J.. Introduction to Molecular Analysis of Ectomycorrhizal Communities[J]. Soil Science Society of America journal,2007,71(2): 601-610 .
APA	MARTIN Kendall J..(2007).Introduction to Molecular Analysis of Ectomycorrhizal Communities.Soil Science Society of America journal,71(2), 601-610 .
MLA	MARTIN Kendall J.."Introduction to Molecular Analysis of Ectomycorrhizal Communities".Soil Science Society of America journal 71.2(2007): 601-610 .