Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil

doi:10.1038/s41598-017-06436-0

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil
	Min, Jun1 ; Wang, Bin 1,2; Hu, Xiaoke1 ; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China)
刊名	SCIENTIFIC REPORTS
	2017-07-20
卷号	7
ISSN号	2045-2322
DOI	10.1038/s41598-017-06436-0
产权排序	第1完成单位
文献子类	Article
英文摘要	para-Nitrophenol (PNP), 3-methyl-4-nitrophenol (3M4NP), and 2-chloro-4-nitrophenol (2C4NP) are highly toxic compounds that have caused serious environmental issues. We inoculated an artificially contaminated soil with Burkholderia sp. strain SJ98, which has the ability to degrade PNP, 3M4NP, and 2C4NP, and quantified bioremediation. There was accelerated degradation of all nitrophenols in inoculated treatments compared to the un-inoculated treatments. The indigenous bacteria were able to degrade PNP, but not 3M4NP or 2C4NP. Real-time PCR targeting the catabolic gene pnpA showed that levels of strain SJ98 remained stable over the incubation period. High-throughput sequencing revealed that both contamination and bioaugmentation influenced the bacterial community structure. Bioaugmentation seemed to protect Kineosporia, Nitrososphaera, and Schlesneria from nitrophenol inhibition, as well as led to a sharp increase in the abundance of Nonomuraea, Kribbella, and Saccharopolyspora. There was a significant increase in the relative abundances of Thermasporomyces, Actinomadura, and Streptomyces in both contaminated and bioaugmented treatments; this indicated that these bacteria are likely directly related to nitrophenol degradation. To our knowledge, this is the first report of the simultaneous removal of PNP, 3M4NP, and 2C4NP using bioaugmentation. This study provides valuable insights into the bioremediation of soils contaminated with nitrophenols.
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WOS关键词	RALSTONIA SP SJ98 ; CONTAMINATED SOIL ; P-NITROPHENOL ; BIOAUGMENTATION ; DIVERSITY ; BIODEGRADATION ; BIOREMEDIATION ; 4-NITROPHENOL ; BIODIVERSITY ; CHEMOTAXIS
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000405907800037
资助机构	National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; Yantai Science and Technology Project(2014ZH088)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/23413]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Hu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China)
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Min, Jun,Wang, Bin,Hu, Xiaoke,et al. Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil[J]. SCIENTIFIC REPORTS,2017,7.
APA	Min, Jun,Wang, Bin,Hu, Xiaoke,&Hu, Xiaoke.(2017).Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil.SCIENTIFIC REPORTS,7.
MLA	Min, Jun,et al."Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil".SCIENTIFIC REPORTS 7(2017).