Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems

doi:10.3389/fmicb.2017.01327

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	Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems
	Liu, Wei 1; Yang, Jinbao 2; Sun, Yu 1,3; Liu, Xiaolin 1,3; Li, Yan 1; Zhang, Zhenpeng 1,3; Xie, Zhihong1 ; Xie, Zhihong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
刊名	FRONTIERS IN MICROBIOLOGY
	2017-07-13
卷号	8
关键词	Chemotaxis Azorhizobium Caulinodans Chemoreceptor Symbiosis Nodulation
ISSN号	1664-302X
DOI	10.3389/fmicb.2017.01327
产权排序	第1完成单位
文献子类	Article
英文摘要	Azorhizobium caulinodans ORS571 is a motile soil bacterium that interacts symbiotically with legume host Sesbania rostrata, forming nitrogen-fixing root and stem nodules. Bacterial chemotaxis plays an important role in establishing this symbiotic relationship. To determine the contribution of chemotaxis to symbiosis in A. caulinodans ORS571-S. rostrata, we characterized the function of TlpA1 (transducer-like protein in A. caulinodans), a chemoreceptor predicted by SMART (Simple Modular Architecture Research Tool), containing two N-terminal transmembrane regions. The tlpA1 gene is located immediately upstream of the unique che gene cluster and is transcriptionally co-oriented. We found that a Delta tlpA1 mutant is severely impaired for chemotaxis to various organic acids, glycerol and proline. Furthermore, biofilm forming ability of the strain carrying the mutation is reduced under certain growth conditions. Interestingly, competitive colonization ability on S. rostrata root surfaces is impaired in the Delta tlpA1 mutant, suggesting that chemotaxis of the A. caulinodans ORS571 contributes to root colonization. We also found that TlpA1 promotes competitive nodulation not only on roots but also on stems of S. rostrata. Taken together, our data strongly suggest that TlpA1 is a transmembrane chemoreceptor involved in A. caulinodans-S. rostrata symbiosis.
URL标识	查看原文
WOS关键词	PROTEIN-SEQUENCE ANALYSES ; RHIZOBIUM-MELILOTI ; SESBANIA-ROSTRATA ; BIOFILM FORMATION ; BACTERIAL CHEMORECEPTORS ; AZOSPIRILLUM-BRASILENSE ; CHEMOTAXIS ; MOTILITY ; SYSTEM ; NODULES
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000406156300001
资助机构	National Natural Science Foundation of China(31370108 ; Chinese Academy of Sciences(KZZD-EW-14) ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Shandong Independent Innovation and Achievement Transformation Pro-gram(2014ZZCX07303) ; Shandong Academy of Agricultural Sciences(+CXGC2016B10) ; Shandong Province Science Foundation(ZR2016CB44) ; 31570063)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/22568]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Xie, Zhihong; Xie, Zhihong(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China 2.Shanxi Agr Univ, Coll Life Sci, Taigu, Peoples R China 3.Univ Chinese Acad Sci, Sch Resource & Environm, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wei,Yang, Jinbao,Sun, Yu,et al. Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA	Liu, Wei.,Yang, Jinbao.,Sun, Yu.,Liu, Xiaolin.,Li, Yan.,...&Xie, Zhihong.(2017).Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems.FRONTIERS IN MICROBIOLOGY,8.
MLA	Liu, Wei,et al."Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems".FRONTIERS IN MICROBIOLOGY 8(2017).