Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa
	Wei, FL; Yu, LX; Li, RH; Zhang, X; Zhang, XH; Zhang, Y; Wang, YQ; Wang, HC; Liang, J; Ma, R
刊名	ANIMAL REPRODUCTION SCIENCE
	2018
卷号	188 页码:13-20
通讯作者	lichangzhong@qhu.edu.cn
英文摘要	The endemic naked carp (Gymnocypris przewalskii Kessler, 1876) plays an important role in the maintenance of the distinctive ecological system of Lake Qinghai at 3.2 km altitude on the Qinghai-Tibet Plateau in China. This study aimed to develop a cryopreservation protocol for Gymnocypris przewalskii spermatozoa. Semen was collected from mature individuals during migration and frozen using the liquid nitrogen vapor method. The influence of different cryoprotectants and three extenders on the post-thaw quality of the sperm was analyzed. The highest sperm motility rate and longest motility time after cryopreservation were achieved by combining Ringer's solution with 15% ethylene glycol (P < 0.05). The fertilization rate of this cryopreserved semen was 15.26 +/- 4.54%. This study thus provides a valuable method for the cryopreservation of the sperm of this important endangered fish species.
学科主题	Agriculture; Reproductive Biology
关键词[WOS]	Semen conservation ; Freezing medium ; Naked carp ; Lake Qinghai
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17029]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Wei, FL,Yu, LX,Li, RH,et al. Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa[J]. ANIMAL REPRODUCTION SCIENCE,2018,188:13-20.
APA	Wei, FL.,Yu, LX.,Li, RH.,Zhang, X.,Zhang, XH.,...&Li, CZ.(2018).Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa.ANIMAL REPRODUCTION SCIENCE,188,13-20.
MLA	Wei, FL,et al."Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa".ANIMAL REPRODUCTION SCIENCE 188(2018):13-20.