Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara | |
Liu, XF; Wu, YH; Wei, SN; Wang, N; Li, PF; Li, YZ; Zhang, NW; Qin, QW; Chen, SL | |
刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
![]() |
2018 | |
卷号 | 44期号:1页码:87-93 |
通讯作者 | chensl@ysfri.ac.cn |
英文摘要 | A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 A degrees C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 A degrees C, with optimal growth at 24 A degrees C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42. Green fluorescent signals could be observed in EMK cells when the cells were transfected with pEGFP-N3 plasmid. Moreover, a significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or nervous necrosis virus (NNV), and viral replication was confirmed by quantitative real-time PCR (qPCR). These results suggested the potential of the EMK cell line for studies of transgene and pathogenesis of SGIV and NNV. |
学科主题 | Biochemistry & Molecular Biology; Fisheries; Physiology |
关键词[WOS] | Kidney cell line ; Epinephelus moara ; Transfection ; SGIV ; NNV |
内容类型 | 期刊论文 |
源URL | [http://ir.scsio.ac.cn/handle/344004/17012] ![]() |
专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
推荐引用方式 GB/T 7714 | Liu, XF,Wu, YH,Wei, SN,et al. Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(1):87-93. |
APA | Liu, XF.,Wu, YH.,Wei, SN.,Wang, N.,Li, PF.,...&Chen, SL.(2018).Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(1),87-93. |
MLA | Liu, XF,et al."Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.1(2018):87-93. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论