Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata
	Zhang, H; Huang, XD; Shi, Y; Liu, WG; He, MX
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018
卷号	73 页码:279-287
通讯作者	hmx2@scsio.ac.cn
英文摘要	The mitogen-activated protein kinase kinase 4 (MKK4) is a key component of the c-Jun N-terminal kinase (JNK) signaling pathway and regulates multiple cellular activities. However, little is known about the roles of this kinase in pearl oyster. In this study, we identified an MKK4 homologue in Pinctada fucata by using a transcriptome database. Sequence analysis and protein structure prediction showed that PfMKK4 is highly conserved to MKK4 from other vertebrate and invertebrate species. Phylogenetic analysis revealed that PfMKK4 has the closest relationship with that from Crassostrea gigas. QPCR was used to investigate expression profiles in different healthy adult tissues and developmental stages of P. fucata. We found that PfMKK4 was ubiquitously expressed in all tissues and developmental stages examined except for in D-shaped larvae. Gene expression analysis suggested that PfMKK4 is involved in the response to the nucleus insertion operation. Lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [poly(I:C)] stimulation in vivo reduced PfMKK4 mRNA expression at 6 h, 48 h and 48 h, 72 h, respectively. LPS and poly(I:C) induced PfMKK4 phosphorylation in a primary mantle cell culture. These results contribute to better understanding of the potential role played by PfMKK4 in protecting the pearl oyster from injury caused by grafting or disease.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
关键词[WOS]	Pinctada fucata ; PfMKK4 ; Gene expression ; Graft ; Immunity
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17007]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhang, H,Huang, XD,Shi, Y,et al. Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73:279-287.
APA	Zhang, H,Huang, XD,Shi, Y,Liu, WG,&He, MX.(2018).Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,73,279-287.
MLA	Zhang, H,et al."Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 73(2018):279-287.