Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals

doi:10.1038/ncomms15587

	Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
	Yu, Tao 2,3; Zhou, Yongjin J.2,3,6; Wenning, Leonie 2,3; Liu, Quanli 2,3; Krivoruchko, Anastasia 2,3,4; Siewers, Verena 2,3; Nielsen, Jens 1,2,3,5; David, Florian 2,3,4
刊名	NATURE COMMUNICATIONS
	2017-05-26
卷号	8
DOI	10.1038/ncomms15587
英文摘要	Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l(-1) in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.
语种	英语
WOS记录号	WOS:000402047300001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152292]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden 2.Chalmers, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden 3.Chalmers, Novo Nordisk Fdn, Ctr Biosustainabil, SE-41296 Gothenburg, Sweden 4.Chalmers, Biopetrolia AB, Syst & Synthet Biol Grp, Kemivagen 10, SE-41296 Gothenburg, Sweden 5.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark 6.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yu, Tao,Zhou, Yongjin J.,Wenning, Leonie,et al. Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals[J]. NATURE COMMUNICATIONS,2017,8.
APA	Yu, Tao.,Zhou, Yongjin J..,Wenning, Leonie.,Liu, Quanli.,Krivoruchko, Anastasia.,...&David, Florian.(2017).Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.NATURE COMMUNICATIONS,8.
MLA	Yu, Tao,et al."Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals".NATURE COMMUNICATIONS 8(2017).