Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure

doi:10.1016/j.carbpol.2017.04.075

	Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure
	Song, Linsheng 3; Xu, Qingsong 3; Wang, Wenjing 4; Qu, Chen 3; Gu, Jianguo 1; Yin, Heng 2; Jia, Zhihao 3; Du, Yuguang 5
刊名	CARBOHYDRATE POLYMERS
	2017-08-15
卷号	170 页码:241-246
关键词	Chitosan Oligosaccharides N-acetylglucosaminyltransferase v Branched N-glycan Cell Migration
DOI	10.1016/j.carbpol.2017.04.075
英文摘要	Chitosan oligosaccharides (COS) have been shown to regulate various cellular and biological functions. The aim of this study was to investigate the antimetastatic potency of COS and the underlying mechanism. Here, we established a stably N-acetylglucosaminyltransferase V (GnT-V)-overexpressed MCF10A cell line. As expected, GnT-V overexpression greatly promoted cell migration in the transfectants by using wound healing assay. However, the induction in the cell migration was significantly suppressed by an addition of COS. Curiously, COS inhibited the protein expression of GnT-V in a dose dependent manner. Consistent with that, the reactivities with datura stramonium (DSA) and leuko-agglutinating phytohemagglutinin (L4-PHA) lectins, which specifically recognize branched N-acetylglucosamine (Gic-NAc) structure, were also suppressed by COS. Taken together, these results demonstrated COS inhibited breast epithelial cell migration through down-regulation of GnT-V and its products, branched N-glycans, indicating that COS may serve as a potential novel therapeutic candidate for the treatment of metastatic breast cancer. (C) 2017 Elsevier Ltd. All rights reserved.
语种	英语
WOS记录号	WOS:000402942900028
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152243]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Tohoku Med & Pharmaceut Univ, Sendai, Miyagi 9818558, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Dalian Ocean Univ, Coll Fisheries & Life Sci, 52 Heishijiao St, Dalian 116023, Peoples R China 4.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Song, Linsheng,Xu, Qingsong,Wang, Wenjing,et al. Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure[J]. CARBOHYDRATE POLYMERS,2017,170:241-246.
APA	Song, Linsheng.,Xu, Qingsong.,Wang, Wenjing.,Qu, Chen.,Gu, Jianguo.,...&Du, Yuguang.(2017).Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure.CARBOHYDRATE POLYMERS,170,241-246.
MLA	Song, Linsheng,et al."Chitosan oligosaccharides inhibit epithelial cell migration through blockade of N-acetylglucosaminyltransferase V and branched GlcNAc structure".CARBOHYDRATE POLYMERS 170(2017):241-246.