A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules

doi:10.1016/j.carbon.2017.05.099

	A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules
	Li, Xin 2,3; Xu, Guiju 2,3; Zhang, Hongyan 2,3; Liu, Shengju 2,3; Niu, Huan 2,3; Peng, Jiaxi 2,3; Wu, Jing 1; Wu, Ren'an 2
刊名	CARBON
	2017-09-01
卷号	121 页码:343-352
关键词	Carbon Nanospheres Mass Spectrometry Surface Assistant Matrix Biomolecules Laser Desorption/ionization
DOI	10.1016/j.carbon.2017.05.099
英文摘要	In this work, a novel strategy for highly efficient detection of small molecules with a homogeneous carbon nanosphere film-spot (HCNFs) was carried out for the first time by negative surface-assisted laser desorption/ionization time-of-flight mass spectroscopy (SALDI-TOF MS). The HCNFs was fabricated by monolayer oxidized carbon nanospheres (OCS) self-assembling on laser desorption/ionization (LDI) target. With the HCNFs, no background interference and exclusive deprotonated ([M-H] ) peaks of analytes were obtained for a wide range of small biomolecules including fatty acids, amino acids, nucleobases, traditional Chinese medicines, and anti-cancer drugs in the negative SALDI-TOF MS. Comparing with other carbon-based matrices as well as the organic matrix, the monolayer OCS film exhibited various advantages including fewer interfering fragments, stronger signal intensities, and higher detection sensitivity. Moreover, with the improved spotting homogeneity of the HCNFs, "sweetspot" searching on LDI target was avoided and good reproducibility was obtained in the LDI-TOF MS analysis. This study is expanding the application of carbon nanospheres and providing a promising approach for rapid analysis of small molecules with none-interference and good reproducibility. (C) 2017 Elsevier Ltd. All rights reserved.
语种	英语
WOS记录号	WOS:000405294400039
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152063]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Wenzhou Inst Biomat & Engn, Wenzhou 325000, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Xu, Guiju,Zhang, Hongyan,et al. A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules[J]. CARBON,2017,121:343-352.
APA	Li, Xin.,Xu, Guiju.,Zhang, Hongyan.,Liu, Shengju.,Niu, Huan.,...&Wu, Ren'an.(2017).A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules.CARBON,121,343-352.
MLA	Li, Xin,et al."A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules".CARBON 121(2017):343-352.