Photoinduced electron transfer from polymer-templated Ag nanoclusters to G-quadruplex-hemin complexes for the construction of versatile biosensors and logic gate applications

doi:10.1007/s00216-018-0887-z

	Photoinduced electron transfer from polymer-templated Ag nanoclusters to G-quadruplex-hemin complexes for the construction of versatile biosensors and logic gate applications
	Qu, Fei 1,2; Mao, Beibei 1,2,3; Xue, Fangfang 1,2; Xia, Lian 1,2; You, Jinmao 1,2,4; Song, Cuihua 1,2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2018-03-01
卷号	410 期号:8 页码:2211-2219
关键词	Ag Nanoclusters G-quadruplex-hemin Complexes Adenosine Triphosphate Target Dna Logic Gate
DOI	10.1007/s00216-018-0887-z
英文摘要	In this paper, fluorescent Ag nanoclusters (Ag NCs) templated by hyperbranched polyethyleneimine (PEI) are utilized as a versatile probe through the photoinduced electron transfer (PET) between PEI-Ag NCs and G-quadruplex-hemin complexes. In the presence of hemin and target molecule, the specific conjugation with its aptamer induces the conformational change of the DNA sequence, releasing the G-quadruplex sequence part. Once the G-quadruplex-hemin complexes are introduced, electron transfer from the PEI-Ag NCs to G-quadruplex-hemin complexes occurs, resulting in fluorescence quenching. Through changing the sensing DNA sequence, this novel PET system enables the specific detection of target DNA and adenosine triphosphate (ATP) with the wide linear range of 1-200 nM and 5-500 nM, respectively, and the corresponding limit of detection as low as 0.3 nM for target DNA and 1.5 nM for ATP. In addition, the proposed method is successfully applied to the determination of ATP in human serum samples with satisfactory recoveries, and a logic gate is fabricated using target molecules and hemin as inputs and the fluorescence signal of PEI-Ag NCs as an output.
语种	英语
WOS记录号	WOS:000426466800015
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13328]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Juye Cty Vocat Secondary Sch, Heze 274000, Shandong, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Qu, Fei,Mao, Beibei,Xue, Fangfang,et al. Photoinduced electron transfer from polymer-templated Ag nanoclusters to G-quadruplex-hemin complexes for the construction of versatile biosensors and logic gate applications[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(8):2211-2219.
APA	Qu, Fei,Mao, Beibei,Xue, Fangfang,Xia, Lian,You, Jinmao,&Song, Cuihua.(2018).Photoinduced electron transfer from polymer-templated Ag nanoclusters to G-quadruplex-hemin complexes for the construction of versatile biosensors and logic gate applications.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(8),2211-2219.
MLA	Qu, Fei,et al."Photoinduced electron transfer from polymer-templated Ag nanoclusters to G-quadruplex-hemin complexes for the construction of versatile biosensors and logic gate applications".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.8(2018):2211-2219.