A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages

doi:10.1016/j.ijbiomac.2017.09.083

	A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages
	Wang, Hailiang 1; Bi, Hongtao 2; Gao, Tingting 2; Zhao, Bin 1; Ni, Weihua 3; Liu, Jun 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-02-01
卷号	107 页码:1039-1045
关键词	Homogalacturonan Hippophae Rhamnoides l Polysaccharide Macrophage Toll-like Receptor 4 Tlr4
DOI	10.1016/j.ijbiomac.2017.09.083
英文摘要	Our previous study isolated a natural high-methoxyl homogalacturonan (HRWP-A) from Hippophae rhamnoides and showed antitumor activity in vivo. In this study, the immunomodulatory activity and mechanisms of action of HRWP-A were further investigated. Results showed that HRWP-A could recover the body condition and activated macrophage in Cyclophosphamide (CTX)-induced immunosuppressed mice. Further, we investigated the possible mechanism underlying the effects of HRWP-A on mouse peritoneal macrophages. qPCR and western blot revealed that HRWP-A upregulated the expression of TLR4 mRNA in vitro. This process was accompanied by a clear increase in MyD88 expression and p-l kappa B-alpha, but these effects were largely abrogated by pretreatment with anti-TLR4 antibodies. The effects of HRWP-A on macrophage NO, IL-1 beta and 1L-6 production were also inhibited by anti-TLR4 antibodies and were greatly influenced by the NF-kappa B inhibitor PDTC. Moreover, HRWP-A failed to induce the production of NO, IL-1 beta and IL-6 in peritoneal macrophages prepared from C3H/HeJ mice, which have a point mutation in the Tlr4 gene, suggesting the involvement of the TLR4 molecule in HRWP-A-mediated macrophage activation. These results may have important implications for our understanding of the structure-activity relationship of immunopotentiating polysaccharides from medicinal herbs. (C) 2017 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000423892200117
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13310]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Jilin Univ, Hosp 2, Dept Neurosurg, Changchun 130021, Jilin, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Qinghai, Peoples R China 3.Jilin Univ, Coll Basic Med Sci, Dept Immunol, Changchun 130033, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hailiang,Bi, Hongtao,Gao, Tingting,et al. A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,107:1039-1045.
APA	Wang, Hailiang,Bi, Hongtao,Gao, Tingting,Zhao, Bin,Ni, Weihua,&Liu, Jun.(2018).A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,107,1039-1045.
MLA	Wang, Hailiang,et al."A homogalacturonan from Hippophae rhamnoides L. Berries enhance immunomodulatory activity through TLR4/MyD88 pathway mediated activation of macrophages".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 107(2018):1039-1045.