Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis

doi:10.1016/j.bios.2015.11.050

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院合肥智能机械研究所

	Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis
	Ye, Lei1 ; Huang, Na-Li 1; Ma, Xue-Ling 2; Schneider, Marion 3; Huang, Xin-Jiu 4; Du, Wei-Dong 3,5
刊名	BIOSENSORS & BIOELECTRONICS
	2016-04-15
卷号	78 期号:无页码:404-410
关键词	Protein Biochip N-succinimidyl 4-(Maleimidomethyl) Cyclohexanecarboxylate (Smcc) Flagellin Outer Surface Protein c (Ospc) Variable Major Protein-like Sequence (Vise) Neuroborreliosis Elisa
DOI	10.1016/j.bios.2015.11.050
文献子类	Article
英文摘要	In this study, we developed a novel protein biochip that was modified with N-succinimidyl 4-(maleimidomethyl)cyclohexanecarboxylate (SMCC) and specialized for concurrent detection of serum IgG and 1gM antibodies against Borrelia burgdorferi antigens, flagellin, outer surface protein C (OspC) and variable major protein-like sequence (VIsE) in the patients with neuroborreliosis (NB), respectively. Surface chemical characteristics of the biochips were validated with atomic force microscope (AFM) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The visualized detection limit for IgG antibodies against flagellin, OspC and VIsE antigens on the biochip were 0.78 mu g/ml, 0.78, mu g/ml and 1.56 mu g/ml, respectively. Finally, serum IgG and IgM antibodies in 72 patients with NB and 188 healthy individuals were tested on the biochip. The seroimmunological outcome by the biochip were evaluated in comparison with enzyme linked immunosorbent assay (ELISA) assay. The results demonstrated that the prevalences of IgG and IgM antibodies in the cases were 41.7%, 63.9% to flagellin; 20.8% and 51.4% to OspC and 76.4%, 62.5% to VIsE, respectively. Utilization of the biochip in detection IgM antibody against flagellin was compatible with ELISA assay (R-2=0.849). Thus, the protein biochip would provide a potential platform not only for enabling detection of corresponding antibodies directed against B. burgdorferi antigens, but also for monitoring course of the disease. (C) 2015 Elsevier B.V. All rights reserved.
WOS关键词	BORRELIA-BURGDORFERI ; LYME BORRELIOSIS ; ERYTHEMA MIGRANS ; DISEASE ; DIAGNOSIS ; VALIDATION ; GOLD ; OSPC
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000368954900057
资助机构	National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; National Natural Science Foundation of China(81171655) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023) ; International Science and Technology cooperation program of Anhui Province(1403062023)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/31583]
专题	合肥物质科学研究院_中科院合肥智能机械研究所
作者单位	1.Anhui Med Univ, Sch Life Sci, Dept Biol, Meishan 81, Hefei 230032, Peoples R China 2.ACURA Kliniken, MVZ, D-76530 Baden Baden, Germany 3.Univ Ulm Klinikum, Sekt Expt Anasthesiol, Albert Einstein Allee 23, D-89081 Ulm, Germany 4.Chinese Acad Sci, Inst Intelligent Machines, Res Ctr Biomimet Funct Mat & Sensing Devices, Hefei 230031, Peoples R China 5.Anhui Med Univ, Dept Pathol, Meishan 81, Hefei 230032, Peoples R China
推荐引用方式 GB/T 7714	Ye, Lei,Huang, Na-Li,Ma, Xue-Ling,et al. Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis[J]. BIOSENSORS & BIOELECTRONICS,2016,78(无):404-410.
APA	Ye, Lei,Huang, Na-Li,Ma, Xue-Ling,Schneider, Marion,Huang, Xin-Jiu,&Du, Wei-Dong.(2016).Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis.BIOSENSORS & BIOELECTRONICS,78(无),404-410.
MLA	Ye, Lei,et al."Establishment of N-succinimidyl 4-(maleimidomethyl) cyclohexanecarboxylate (SMCC) modified biochip enabling concurrent detection of serum infectious antibodies in neuroborreliosis".BIOSENSORS & BIOELECTRONICS 78.无(2016):404-410.