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	Synthesis of L- and D-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization
	Huang, Yi-Chao1,2,3; Chen, Chen-Chen4; Gao, Shuai3; Wang, Ye-Hai1,2; Xiao, Hua1,2; Wang, Feng3; Tian, Chang-Lin4; Li, Yi-Ming1,2
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2016-05-23
卷号	22期号:22页码:7623-7628
关键词	Desulfurization Methyl Thioglycolate Native Chemical Ligation Proteins X-ray Diffraction
DOI	10.1002/chem.201600101
文献子类	Article
英文摘要	Native chemical ligation combined with desulfurization has become a powerful strategy for the chemical synthesis of proteins. Here we describe the use of a new thiol additive, methyl thioglycolate, to accomplish one-pot native chemical ligation and metal-free desulfurization for chemical protein synthesis. This one-pot strategy was used to prepare ubiquitin from two or three peptide segments. Circular dichroism spectroscopy and racemic protein X-ray crystallography confirmed the correct folding of ubiquitin. Our results demonstrate that proteins synthesized chemically by streamlined 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis coupled with a one-pot ligation-desulfurization strategy can supply useful molecules with sufficient purity for crystallographic studies.
WOS关键词	NATIVE CHEMICAL LIGATION ; EXPRESSED PROTEIN LIGATION ; PHASE PEPTIDE-SYNTHESIS ; PROLINE LIGATION ; HIGHLY EFFICIENT ; CHEMISTRY ; HYDRAZIDES ; THIOESTER ; ERYTHROPOIETIN ; DESELENIZATION
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000379727900041
资助机构	NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; NSFC(21372058 ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; State Key Laboratory of Medicinal Chemical Biology ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21572043 ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034) ; 21302034)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22492]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Hefei Univ Technol, Sch Med Engn, Hefei 230009, Peoples R China 2.Nankai Univ, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China 3.Tsinghua Univ, Dept Chem, Sch Life Sci, Beijing 100084, Peoples R China 4.Chinese Acad Sci, High Field Magnet Lab, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yi-Chao,Chen, Chen-Chen,Gao, Shuai,et al. Synthesis of L- and D-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization[J]. CHEMISTRY-A EUROPEAN JOURNAL,2016,22(22):7623-7628.
APA	Huang, Yi-Chao.,Chen, Chen-Chen.,Gao, Shuai.,Wang, Ye-Hai.,Xiao, Hua.,...&Li, Yi-Ming.(2016).Synthesis of L- and D-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization.CHEMISTRY-A EUROPEAN JOURNAL,22(22),7623-7628.
MLA	Huang, Yi-Chao,et al."Synthesis of L- and D-Ubiquitin by One-Pot Ligation and Metal-Free Desulfurization".CHEMISTRY-A EUROPEAN JOURNAL 22.22(2016):7623-7628.

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