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	Hmb(off/on) as a switchable thiol protecting group for native chemical ligation
	Qi, Yun-Kun1,2; Tang, Shan1; Huang, Yi-Chao1; Pan, Man1; Zheng, Ji-Shen2; Liu, Lei1
刊名	ORGANIC & BIOMOLECULAR CHEMISTRY
	2016
卷号	14期号:18页码:4194-4198
DOI	10.1039/c6ob00450d
文献子类	Article
英文摘要	A new thiol protecting group Hmb(off/on) is described, which has a switchable activity that may be useful in the chemical synthesis of proteins. When placed on the side chain of Cys, Cys(Hmb(off)) is stable to trifluoroacetic acid (TFA) in the process of solid-phase peptide synthesis. When Cys(Hmb(off)) is treated with neutral aqueous buffers, it is cleanly converted to acid-labile Cys(Hmb(on)), which can later be fully deprotected by TFA to generate free Cys. The utility of Cys(Hmb(off/on)) is demonstrated by the chemical synthesis of an erythropoietin segment, EPO[Cys(98)-Arg(166)]-OH through native chemical ligation.
WOS关键词	PHASE PEPTIDE-SYNTHESIS ; PROTEIN-SYNTHESIS ; CHEMOSELECTIVE LIGATION ; CYSTEINE RESIDUES ; DESULFURIZATION ; CHEMISTRY ; HYDRAZIDES ; CONDENSATION ; BOND ; POLYPEPTIDES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000375610600004
资助机构	National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; National Basic Research Program of China (973 program)(2013CB932800) ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; Natural Science Foundation of Anhui Province(1508085QB30) ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206) ; 21402206)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22399]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ,Dept Chem, Beijing 100084, Peoples R China 2.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Qi, Yun-Kun,Tang, Shan,Huang, Yi-Chao,et al. Hmb(off/on) as a switchable thiol protecting group for native chemical ligation[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2016,14(18):4194-4198.
APA	Qi, Yun-Kun,Tang, Shan,Huang, Yi-Chao,Pan, Man,Zheng, Ji-Shen,&Liu, Lei.(2016).Hmb(off/on) as a switchable thiol protecting group for native chemical ligation.ORGANIC & BIOMOLECULAR CHEMISTRY,14(18),4194-4198.
MLA	Qi, Yun-Kun,et al."Hmb(off/on) as a switchable thiol protecting group for native chemical ligation".ORGANIC & BIOMOLECULAR CHEMISTRY 14.18(2016):4194-4198.

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