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	Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing
	Lu, Yong1; Guo, Zheng2; Song, Jing-Jing1; Huang, Qin-An1; Zhu, Si-Wei1; Huang, Xing-Jiu2; Wei, Yan1
刊名	ANALYTICA CHIMICA ACTA
	2016-01-28
卷号	905期号:无页码:58-65
关键词	Tunable Nanogap Devices Streptavidin Electrochemical Impedance Ultra-sensitive Electron-transfer Resistance
DOI	10.1016/j.aca.2015.11.036
文献子类	Article
英文摘要	A wealth of research has been available discussing nanogap devices for detecting very small quantities of biomolecules by observing their electrical behavior generally performed in dry conditions. We report that a gold nanogapped electrode with tunable gap length for ultra-sensitive detection of streptavidin based on electrochemical impedance technique. The gold nanogap is fabricated using simple monolayer film deposition and in-situ growth of gold nanoparticles in a traditional interdigitated array (IDA) microelectrode. The electrochemical impedance biosensor with a 25-nm nanogap is found to be ultra-sensitive to the specific binding of streptavidin to biotin. The binding of the streptavidin hinder the electron transfer between two electrodes, resulting in a large increase in electron-transfer resistance (R-et) for operating the impedance. A linear relation between the relative R-et and the logarithmic value of streptavidin concentration is observed in the concentration range from 1 pM (picomolar) to 100 nM (nanomolar). The lowest detectable concentration actually measured reaches 1 pM. We believe that such an electrochemical impedance nanogap biosensor provides a useful approach towards biomolecular detection that could be extended to a number of other systems. (C) 2015 Elsevier B.V. All rights reserved.
WOS关键词	GOLD PARTICLE FILM ; ESCHERICHIA-COLI O157-H7 ; ELECTRICAL DETECTION ; NANOPARTICLE ARRAY ; DNA-HYBRIDIZATION ; SENSOR ARRAY ; IMMUNOSENSOR ; ELECTRODE ; SPECTROSCOPY ; TRANSPORT
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000367395000006
资助机构	National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National Key Scientific Program-Nanoscience and Nanotechnology(2011CB933700) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; National natural Science Foundation of China(21105073) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; Project of Educational Committee of Anhui Province(KJ2010B240) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201307) ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; University outstanding youth talent support program of Anhui Province ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008) ; CAS Institute of Physical Science, University of Science and Technology of China(2012FXCX008)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22225]
专题	合肥物质科学研究院_中科院合肥智能机械研究所
作者单位	1.Wannan Med Coll, Dept Chem, Wuhu 241002, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Nanomat & Environm Detect Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Lu, Yong,Guo, Zheng,Song, Jing-Jing,et al. Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing[J]. ANALYTICA CHIMICA ACTA,2016,905(无):58-65.
APA	Lu, Yong.,Guo, Zheng.,Song, Jing-Jing.,Huang, Qin-An.,Zhu, Si-Wei.,...&Wei, Yan.(2016).Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing.ANALYTICA CHIMICA ACTA,905(无),58-65.
MLA	Lu, Yong,et al."Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing".ANALYTICA CHIMICA ACTA 905.无(2016):58-65.

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