Accurate quantification of water-macromolecule exchange induced frequency shift: Effects of reference substance

doi:10.1002/mrm.24223

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	Accurate quantification of water-macromolecule exchange induced frequency shift: Effects of reference substance
	Leutritz, Tobias 1; Hilfert, Liane 2; Smalla, Karl-Heinz 3; Speck, Oliver 1; Zhong, Kai 1,4
刊名	MAGNETIC RESONANCE IN MEDICINE
	2013
卷号	69 期号:1 页码:263-268
关键词	Nmr Reference Tmsp Dioxane Water-macromolecule Exchange Phase Contrast
DOI	10.1002/mrm.24223
文献子类	Article
英文摘要	Watermacromolecule exchange induces a bulk water frequency shift contributing to the contrast in phase imaging. For separating the effects of the watermacromolecule exchange and the macromolecule susceptibility, appropriate internal or external references are needed. In this study, two internal reference compounds, 2,2,3,3-tetradeuterio-3-trimethylsilyl-propionate (TMSP) and 1,4-dioxane, were used to study the macromolecule-dependent water frequency shift in a bovine serum albumin (BSA)water system in detail. For TMSP, the watermacromolecule exchange shift depended on both the BSA and the reference concentration and stabilized to a value of 0.025 ppm/mM (298 K, TMSP concentrations > 30 mM). For dioxane, the dependency of the watermacromolecule exchange shift on the BSA concentration is independent of dioxane at low concentrations. The resulting shift was smaller (0.009 ppm/mM) when compared with using higher TMSP concentrations as reference. This discrepancy might be due to additional dioxanewater interactions. Measurements with an external chloroform reference in a coaxial geometry showed a shift of -0.013 ppm/mM resulting from the opposing effects of macromolecules in water exchange-induced shift and diamagnetic susceptibility shift. All these effects should be considered in the interpretation of tissue phase contrast. From the experimental data, the equilibrium binding constant between BSA and TMSP has been quantified to be Kd = 1.3 +/- 0.4, and the estimated number of interaction sites for BSA is 12.7 +/- 2.6. Magn Reson Med, 2013. (c) 2012 Wiley Periodicals, Inc.
WOS关键词	PHASE ; SUSCEPTIBILITY ; CONTRAST ; BRAIN
WOS研究方向	Radiology, Nuclear Medicine & Medical Imaging
语种	英语
WOS记录号	WOS:000312725500030
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22203]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Otto Von Guericke Univ, Dept Biomed Magnet Resonance, D-39120 Magdeburg, Germany 2.Otto Von Guericke Univ, Inst Chem, D-39120 Magdeburg, Germany 3.Leibniz Inst Neurobiol, Magdeburg, Germany 4.Chinese Acad Sci, High Field Magnet Lab, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Leutritz, Tobias,Hilfert, Liane,Smalla, Karl-Heinz,et al. Accurate quantification of water-macromolecule exchange induced frequency shift: Effects of reference substance[J]. MAGNETIC RESONANCE IN MEDICINE,2013,69(1):263-268.
APA	Leutritz, Tobias,Hilfert, Liane,Smalla, Karl-Heinz,Speck, Oliver,&Zhong, Kai.(2013).Accurate quantification of water-macromolecule exchange induced frequency shift: Effects of reference substance.MAGNETIC RESONANCE IN MEDICINE,69(1),263-268.
MLA	Leutritz, Tobias,et al."Accurate quantification of water-macromolecule exchange induced frequency shift: Effects of reference substance".MAGNETIC RESONANCE IN MEDICINE 69.1(2013):263-268.