Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium | |
Liu, Lei1; Li, Ping1; Qin, Ge1; Yan, Yue1; Li, Yi1; Yao, JianMing2; Wang, Hailei1,3 | |
刊名 | BIORESOURCES |
2016-11-01 | |
卷号 | 11期号:4页码:9940-9955 |
关键词 | Ethanol Phanerochaete Chrysosporium Alcohol Dehydrogenase Mn-dependent Peroxidase Cellulase |
DOI | 10.15376/biores.11.4.9940-9955 |
文献子类 | Article |
英文摘要 | The potential of Phanerochaete chrysosporium in ethanol fermentation was evaluated. During the initial submerged cultivation, 1.76 g/L ethanol was obtained using glucose as substrate. After mutation, the ethanol concentration of an alcohol dehydrogenase (ADH) mutant reached 5.02 g/L. Both base transition and nine-base frame shift mutation occurred in the ADH gene of the mutant, changing the secondary and tertiary structures of ADH, as well as increasing the ADH activity during cultivation. Moreover, P. chrysosporium converted corn stalk to ethanol by a one-step process. After statistical optimizations, 0.26 g/g center dot substrate of ethanol yield was obtained on day 10. During the fermentation, the maximum lignin peroxidase, Mn-dependent peroxidase, and cellulase activities were 29.0 U/L, 256.5 U/L, and 40 U/mL, respectively, thus explaining why the fungus directly ferments corn stalk to ethanol. This study is the first report of the conversion of corn stalk without pretreatment to ethanol using a white-rot fungus. |
WOS关键词 | WHEAT-STRAW ; SACCHAROMYCES-CEREVISIAE ; CATABOLITE REPRESSION ; FUEL ETHANOL ; FERMENTATION ; LIGNIN ; PRETREATMENT ; FEEDSTOCKS ; DISRUPTION ; CELLULASE |
WOS研究方向 | Materials Science |
语种 | 英语 |
WOS记录号 | WOS:000391801300135 |
资助机构 | National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; 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Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/21707] |
专题 | 合肥物质科学研究院_中科院等离子体物理研究所 |
作者单位 | 1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 2.Chinese Acad Sci, Inst Plasma Phys, Hefei 230031, Peoples R China 3.Univ Henan Prov, Key Lab Microorganisms & Funct Mol, Xinxiang 453007, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Lei,Li, Ping,Qin, Ge,et al. Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium[J]. BIORESOURCES,2016,11(4):9940-9955. |
APA | Liu, Lei.,Li, Ping.,Qin, Ge.,Yan, Yue.,Li, Yi.,...&Wang, Hailei.(2016).Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium.BIORESOURCES,11(4),9940-9955. |
MLA | Liu, Lei,et al."Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium".BIORESOURCES 11.4(2016):9940-9955. |
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