Identification, characterization and in vitro neuroprotection of N-6-(4-hydroxybenzyl) adenine riboside and its metabolites

	Identification, characterization and in vitro neuroprotection of N-6-(4-hydroxybenzyl) adenine riboside and its metabolites
	Wang, Li 3; Wang, Jialing 2; Tang, Chunlan 2,3; Xiao, Hongbin 1; Zhao, Jinshun 2; Cui, Wei 2; Cheng, Mengchun 3; Yu, Jie 2
刊名	PHYTOCHEMISTRY LETTERS
	2017-06-01
卷号	20 页码:146-150
关键词	N-6-(4-hydroxybenzyl) adenine riboside Metabolism Neuroprotection
英文摘要	N-6-(4-hydroxybenzyl) adenine riboside (NHBA), isolated from Gastrodia elata Blume, has been demonstrated to show great pharmacological effects. The present study aimed to synthesize and identify the metabolites of NHBA, and to determine their neuroprotective potentials in vitro. After incubation with rat liver microsomes in the presence of NADPH, two metabolites were detected, which were further semisynthesized and identified as N-6-(4-hydroxylbenzyl) purine (NHBP) and N-6-(3,4-dihydroxylbenzyl) adenine riboside (ONHBA) by UPLC-QTOF-MS, H-1 NMR and C-13 NMR. Furthermore, the neuroprotective activities of NHBA and two metabolites were evaluated in SH-SY5Y cells. Our results demonstrated that NHBA substantially protected against H2O2-induced neuronal death in SH-SY5Y cells. Moreover, both ONHBA and NHBP could significantly prevent A beta oligomers-and H2O2-induced neuronal death in SH-SY5Y cells. These results suggested that NHBA and its metabolites, ONHBA and NHBP, might be suitable for the development of new drugs in the treatment of neurodegenerative diseases, including Alzheimer's disease in particular.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences ; Chemistry, Medicinal
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	GASTRODIA-ELATA BL ; CELLS ; PROTECTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000405467600029
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/151901]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Beijing Univ Chinese Med, Beijing 100029, Peoples R China 2.Ningbo Univ, Zhejiang Prov Key Lab Pathol & Physiol Technol, Dept Prevent Med, Med Sch, Ningbo 315211, Zhejiang, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Wang, Jialing,Tang, Chunlan,et al. Identification, characterization and in vitro neuroprotection of N-6-(4-hydroxybenzyl) adenine riboside and its metabolites[J]. PHYTOCHEMISTRY LETTERS,2017,20:146-150.
APA	Wang, Li.,Wang, Jialing.,Tang, Chunlan.,Xiao, Hongbin.,Zhao, Jinshun.,...&Yu, Jie.(2017).Identification, characterization and in vitro neuroprotection of N-6-(4-hydroxybenzyl) adenine riboside and its metabolites.PHYTOCHEMISTRY LETTERS,20,146-150.
MLA	Wang, Li,et al."Identification, characterization and in vitro neuroprotection of N-6-(4-hydroxybenzyl) adenine riboside and its metabolites".PHYTOCHEMISTRY LETTERS 20(2017):146-150.