Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment

	Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment
	Li Xiu-Ling 1; Chen Cheng 1; Wang Hong-Xi 2; Kang Hong-Jian 1; Yao Yao 2; Qing Guang-Yan 2; Liang Xin-Miao 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2017-08-01
卷号	45 期号:8 页码:1149-1154
关键词	Hydrophilic interaction chromatography Tetrapeptide Glycopeptide enrichment Mass spectrometry
英文摘要	In this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed. The tetrapeptide modified silica gel materials (denoted as Poly. DAPD) were synthesized by atom. transfer radical. polymerization (ATRP) and characterized by N-2 adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X. ray photoelectron spectrometer (XPS). The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel. Poly. DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid. phase extraction (SPE) mode. Comparing with commercialized ZIC. HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as. prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti. interference property. The SPE results demonstrated that the tetrapeptide. based HILIC materials could be a potential tool in large. scale glycosylation analysis.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	N-LINKED GLYCOPEPTIDES ; SOLID-PHASE EXTRACTION ; MASS-SPECTROMETRY ; GLYCOSYLATION ; GLYCOPROTEINS ; HETEROGENEITY ; FETUIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000414612900006
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149737]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li Xiu-Ling,Chen Cheng,Wang Hong-Xi,et al. Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(8):1149-1154.
APA	Li Xiu-Ling.,Chen Cheng.,Wang Hong-Xi.,Kang Hong-Jian.,Yao Yao.,...&Liang Xin-Miao.(2017).Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(8),1149-1154.
MLA	Li Xiu-Ling,et al."Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High. efficiency Glycopeptide Enrichment".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.8(2017):1149-1154.