Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling | |
Deng Yu-Lin1; Zheng Nai-Ren1; Shan Yi-Chu2; Zhang Yu-Kui1,2 | |
刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY |
2017-10-01 | |
卷号 | 45期号:10页码:1441-1447 |
关键词 | Proteomics Isobaric labeling Identification of proteins |
英文摘要 | Isobaric peptide termini labeling (IPTL) is a technology which uses light and heavy isotopes to label C-terminus and N-terminus of peptides. As the masses of labeled peptides are equivalent, the complexity of sample is low when analyzing MS data produced by this technology. Besides, paired b and y ions are helpful while analyzing MS/MS data in this kind of experiments. On the basis of this, a novel scoring algorithm, all ions scoring algorithm (AISA), has been designed for IPTL experiments. The information of quantification and qualification can be acquired at the same time using AISA. On Q-Exactive HeLa 2D RPLC dataset, peptide spectrum matches (PSMs), distinct peptides and protein groups identified by AISA are 15%, 26% and 22% higher than Morpheus. On human-HCC-HL dataset, PSMs, distinct peptides and protein groups identified by AISA are 24%, 39% and 27% higher than Morpheus. Quantification ratio on Q-Exactive HeLa and human. HCC-HL datasets are 1.18 and 0.90, respectively, which are very close to 1. Besides, quantification ratios between 0.5 and 2.0 are 91% and 94%, respectively. |
WOS标题词 | Science & Technology ; Physical Sciences |
类目[WOS] | Chemistry, Analytical |
研究领域[WOS] | Chemistry |
关键词[WOS] | TANDEM MASS TAGS ; QUANTITATIVE PROTEOMICS ; MS/MS ; SPECTROMETRY ; EXPRESSION ; MIXTURES |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000414957900004 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149672] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
推荐引用方式 GB/T 7714 | Deng Yu-Lin,Zheng Nai-Ren,Shan Yi-Chu,et al. Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(10):1441-1447. |
APA | Deng Yu-Lin,Zheng Nai-Ren,Shan Yi-Chu,&Zhang Yu-Kui.(2017).Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(10),1441-1447. |
MLA | Deng Yu-Lin,et al."Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.10(2017):1441-1447. |
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