A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine

	A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine
	You, Jinmao1,2,3 ; Qu, Fei 1,2; Liu, Yining 1,2; Kong, Rongmei 1,2
刊名	MICROCHIMICA ACTA
	2017-11-01
卷号	184 期号:11 页码:4417-4424
关键词	Fluorescence Electrolytes Aggregation ssDNA Complementary oligonucleotide Hepatitis B virus Dopamine Dopamine-binding aptamer
英文摘要	The authors report that the fluorescence of polymer-capped silver nanoclusters (AgNCs) is quenched by MoS2 nanosheets. However, in the presence of electrolytes such as sodium acetate (50 mM), the nanosheets tend to aggregate, and this causes fluorescence to be restored. If, however, ssDNA is added, it will be adsorbed on the surface of the MoS2 nanosheets and stabilize them against aggregation. As a result, the emission of the AgNCs (best measured at excitation/emission wavelengths of 375/455 nm) is quenched again. If complementary oligonucleotide is added, a dsDNA will be formed that does no longer prevent aggregation and fluorescence is restored. Similarly, if an aptamer is adsorbed on the MoS2 nanosheets and its substrate is then added to form a folded state, the fluorescence of the AgNCs also will be restored. As a proof of concept, the DNA sequences of hepatitis B virus and of a dopamine-selective aptamer were chosen as models. The resulting assays have low detection limits and linear ranges that extend from 5 to 30 nM for hepatitis B DNA, and from 0.3 to 1 mu M for dopamine. In our perception, this method represents a universal platform for the detection of analytes for which complementary oligomers or aptamers are available.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	GOLD NANOPARTICLES ; LABEL-FREE ; ELECTRODE ; POLYETHYLENEIMINE ; BIOSENSOR ; RECOVERY ; PLATFORM ; IONS ; ACID ; DOTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000412901100023
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/9826]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	You, Jinmao,Qu, Fei,Liu, Yining,et al. A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine[J]. MICROCHIMICA ACTA,2017,184(11):4417-4424.
APA	You, Jinmao,Qu, Fei,Liu, Yining,&Kong, Rongmei.(2017).A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine.MICROCHIMICA ACTA,184(11),4417-4424.
MLA	You, Jinmao,et al."A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine".MICROCHIMICA ACTA 184.11(2017):4417-4424.