A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe

	A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe
	Wang, Hua 1; Lei, Cuihua 1; Zhao, Xian-En 1,2; Sun, Jing2 ; Yan, Xiaolu 1; Gao, Yue 1; Gao, Han 1; Zhu, Shuyun 1,2
刊名	TALANTA
	2017-12-01
卷号	175 页码:457-462
关键词	Silver ion (Ag+) 3,3 ',5,5 '-tetramethylbenzidine (TMB) Tyrosinase Enzyme inhibition Colorimetric assay
英文摘要	A novel colorimetric method for the detection of tyrosinase (TYR) and its inhibitor by taking utilization of Ag+-3,3 ',5,5 '-tetramethylbenzidine (TMB) detection system has been proposed. Ag+ could oxidize TMB to oxidized TMB (oxTMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm. The addition of dopamine (DA) could cause the reduction of oxTMB which resulted in the fading of the blue color and a decrease of the absorbance at 652 nm. However, in the presence of TYR, DA could be oxidized to dopaquinone, which inhibited the reduction of oxTMB by DA, resulting in a blue color recovery and an increase of the absorbance at 652 nm. Based on this finding, we propose a method to quantitatively detect TYR activity with the help of UV-vis spectroscopy. The developed assay is highly sensitive with a low detection limit of 0.010 U/mL. More importantly, this method is fairly simple and inexpensive without the use of complicated nanomaterials. In addition, it constructs a useful platform for TYR inhibitor screening.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	QUANTUM DOTS ; BIOCATALYTIC TRANSFORMATIONS ; MUSHROOM TYROSINASE ; GOLD NANOCLUSTERS ; DUAL-EMISSION ; IN-VITRO ; DOPAMINE ; NANOPARTICLES ; GLUTATHIONE ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000412033100060
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/9810]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu City 273165, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hua,Lei, Cuihua,Zhao, Xian-En,et al. A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe[J]. TALANTA,2017,175:457-462.
APA	Wang, Hua.,Lei, Cuihua.,Zhao, Xian-En.,Sun, Jing.,Yan, Xiaolu.,...&Zhu, Shuyun.(2017).A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe.TALANTA,175,457-462.
MLA	Wang, Hua,et al."A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe".TALANTA 175(2017):457-462.