Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique

	Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique
	Pei, Jinjin 2; Jiang, Lei 1
刊名	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
	2017-07-01
卷号	50 期号:1 页码:41-46
关键词	Andrias davidianus Mucus Antimicrobial peptide Magnetic cell membrane separation Escherichia coli
英文摘要	Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described from A. davidianus until now. Here we describe a novel AMP (andricin 01) isolated from the mucus of A. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
关键词[WOS]	CHINESE ; CHROMATOGRAPHY ; SKINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000405983100007
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/7060]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhong 723001, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Pei, Jinjin,Jiang, Lei. Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique[J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,2017,50(1):41-46.
APA	Pei, Jinjin,&Jiang, Lei.(2017).Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,50(1),41-46.
MLA	Pei, Jinjin,et al."Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique".INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 50.1(2017):41-46.