A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.

doi:10.1016/j.jviromet.2016.07.015

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	A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.
	Liu, Tianfu; Wan, Zhenzhou; Liu, Jia; Zhang, Lingyi; Zhou, Yanheng; Lan, Ke; Hu, Yihong; Zhang, Chiyu; Hu, YH; Zhang, CY (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, CAS Key Lab Mol Virol & Immunol, Yueyang Rd 320, Shanghai 200031, Peoples R China.
刊名	Journal of virological methods
	2016-10
卷号	236 页码:132-138
关键词	HCV Viral load Real-time RT-PCR Partially double-stranded linear DNA probe
ISSN号	1879-0984
DOI	10.1016/j.jviromet.2016.07.015
文献子类	Article
英文摘要	The detection and quantification of HCV RNA is very helpful for the management and treatment of HCV related diseases. Detection of low HCV viral load is a great challenge in HCV RNA detection. Here, we developed a novel real-time RT-PCR assay with partially double-stranded linear DNA probe which can detect all HCV genotypes and improve the detection performance. The novel assay has a wide linear dynamic range of HCV RNA quantification (1 x 10(2)-1 x 10(11)IU/ml) and a limit of detection of 78 The assay exhibits an excellent reproducibility with 2.52% and 1.33% coefficients of variations, for inter and intra-assays, respectively. To evaluate the viability of the assay, a comparison with a commercial HCV RNA detection kit was performed using 106 serum samples. The lineared correlation coefficient between the novel assay and the commercial HCV RNA detection kit was 0.940. Meanwhile, the deviation between the two methods was tolerable. Therefore, the novel real-time RT-PCR assay was applicable for laboratory diagnosis and monitoring of HCV infection. (C) 2016 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
出版地	AMSTERDAM
资助项目	国家科技支撑计划
项目编号	development fund for Shanghai talents ; Science and Technology Support Foundations of Taizhou City
语种	英语
WOS记录号	WOS:000383941900021
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179466]
专题	第二临床医学院_期刊论文
通讯作者	Hu, YH; Zhang, CY (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, CAS Key Lab Mol Virol & Immunol, Yueyang Rd 320, Shanghai 200031, Peoples R China.
推荐引用方式 GB/T 7714	Liu, Tianfu,Wan, Zhenzhou,Liu, Jia,et al. A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.[J]. Journal of virological methods,2016,236:132-138.
APA	Liu, Tianfu.,Wan, Zhenzhou.,Liu, Jia.,Zhang, Lingyi.,Zhou, Yanheng.,...&Zhang, CY .(2016).A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA..Journal of virological methods,236,132-138.
MLA	Liu, Tianfu,et al."A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.".Journal of virological methods 236(2016):132-138.