A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA. | |
Liu, Tianfu; Wan, Zhenzhou; Liu, Jia; Zhang, Lingyi; Zhou, Yanheng; Lan, Ke; Hu, Yihong; Zhang, Chiyu; Hu, YH; Zhang, CY (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, CAS Key Lab Mol Virol & Immunol, Yueyang Rd 320, Shanghai 200031, Peoples R China. | |
刊名 | Journal of virological methods
![]() |
2016-10 | |
卷号 | 236页码:132-138 |
关键词 | HCV Viral load Real-time RT-PCR Partially double-stranded linear DNA probe |
ISSN号 | 1879-0984 |
DOI | 10.1016/j.jviromet.2016.07.015 |
文献子类 | Article |
英文摘要 | The detection and quantification of HCV RNA is very helpful for the management and treatment of HCV related diseases. Detection of low HCV viral load is a great challenge in HCV RNA detection. Here, we developed a novel real-time RT-PCR assay with partially double-stranded linear DNA probe which can detect all HCV genotypes and improve the detection performance. The novel assay has a wide linear dynamic range of HCV RNA quantification (1 x 10(2)-1 x 10(11)IU/ml) and a limit of detection of 78 The assay exhibits an excellent reproducibility with 2.52% and 1.33% coefficients of variations, for inter and intra-assays, respectively. To evaluate the viability of the assay, a comparison with a commercial HCV RNA detection kit was performed using 106 serum samples. The lineared correlation coefficient between the novel assay and the commercial HCV RNA detection kit was 0.940. Meanwhile, the deviation between the two methods was tolerable. Therefore, the novel real-time RT-PCR assay was applicable for laboratory diagnosis and monitoring of HCV infection. (C) 2016 Elsevier B.V. All rights reserved. |
学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
出版地 | AMSTERDAM |
资助项目 | 国家科技支撑计划 |
项目编号 | development fund for Shanghai talents ; Science and Technology Support Foundations of Taizhou City |
语种 | 英语 |
WOS记录号 | WOS:000383941900021 |
资助机构 | MOST |
内容类型 | 期刊论文 |
源URL | [http://ir.lzu.edu.cn/handle/262010/179466] ![]() |
专题 | 第二临床医学院_期刊论文 |
通讯作者 | Hu, YH; Zhang, CY (reprint author), Chinese Acad Sci, Inst Pasteur Shanghai, Pathogen Diagnost Ctr, CAS Key Lab Mol Virol & Immunol, Yueyang Rd 320, Shanghai 200031, Peoples R China. |
推荐引用方式 GB/T 7714 | Liu, Tianfu,Wan, Zhenzhou,Liu, Jia,et al. A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.[J]. Journal of virological methods,2016,236:132-138. |
APA | Liu, Tianfu.,Wan, Zhenzhou.,Liu, Jia.,Zhang, Lingyi.,Zhou, Yanheng.,...&Zhang, CY .(2016).A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA..Journal of virological methods,236,132-138. |
MLA | Liu, Tianfu,et al."A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA.".Journal of virological methods 236(2016):132-138. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论