Anti-nephrolithic potential of catechin in melamine-related urolithiasis via the inhibition of ROS, apoptosis, phospho-p38, and osteopontin in male Sprague-Dawley rats

doi:10.3109/10715762.2015.1061187

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	Anti-nephrolithic potential of catechin in melamine-related urolithiasis via the inhibition of ROS, apoptosis, phospho-p38, and osteopontin in male Sprague-Dawley rats
	Li, X; Wu, G; Shang, P; Bao, J; Lu, J; Yue, Z; Yue, Z (reprint author), Lanzhou Univ, Hosp 2, Gansu Nephrourol Clin Ctr, Inst Urol,Dept Urol, 88 Cui Ying Men, Lanzhou 730030, Gansu, Peoples R China.
刊名	FREE RADICAL RESEARCH
	2015
卷号	49 期号:10 页码:1249-1258
关键词	urolithiasis melamine catechin osteopontin apoptosis
ISSN号	1071-5762
DOI	10.3109/10715762.2015.1061187
文献子类	Article
英文摘要	The addition of melamine to infant formula may cause urolithiasis in humans and animals. This study examined the effects of catechin, an antioxidant, on melamine -cyanuric acid mixture (MCM)-induced crystallization in vitro and in vivo. In an in vitro study, crystal formation induced by an MCM was evaluated in media under various pH conditions and with catechin co-treatment. In an in vivo study, rats were administered an MCM (400 mg/kg, 1: 1, via oral feeding tube) for four weeks and co-treated with catechin, after which crystal formation was observed. Oxidative stress biomarkers and nephrotoxicity were measured. Apoptotic cells were examined using the TUNEL assay. Phosphop38 and osteopontin were evaluated via immunohistochemistry and Western blotting. MCM-induced crystal formation was pH-dependent in conditioned media, and catechin reduced the overall number of crystals. In the in vivo study, catechin suppressed MCM-induced protein expression and apoptosis in rats. Catechin consistently reduced the MCM-mediated production of renal malondialdehyde (MDA) and urinary 8-isoprostane (8-IP) in MCM-treated rats. The activities of antioxidant enzymes such as superoxide dismutase (SOD) were enhanced by catechin. Catechin consistently and significantly reduced levels of renal crystals and nephrotoxicity. Our findings suggest that catechin exhibits anti-nephrolithic potential by chemically inhibiting the formation of crystals and by inhibiting reactive oxygen species, apoptosis, phospho-P38, and osteopontin signaling in rats.
学科主题	Biochemistry & Molecular Biology
出版地	ABINGDON
资助项目	甘肃省杰出青年基金计划
项目编号	Gansu Province Outstanding Youth Science Fund Project [1111RJDA0001]
语种	英语
WOS记录号	WOS:000361329500008
资助机构	GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179311]
专题	第二临床医学院_期刊论文
通讯作者	Yue, Z (reprint author), Lanzhou Univ, Hosp 2, Gansu Nephrourol Clin Ctr, Inst Urol,Dept Urol, 88 Cui Ying Men, Lanzhou 730030, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Li, X,Wu, G,Shang, P,et al. Anti-nephrolithic potential of catechin in melamine-related urolithiasis via the inhibition of ROS, apoptosis, phospho-p38, and osteopontin in male Sprague-Dawley rats[J]. FREE RADICAL RESEARCH,2015,49(10):1249-1258.
APA	Li, X.,Wu, G.,Shang, P.,Bao, J.,Lu, J.,...&Yue, Z .(2015).Anti-nephrolithic potential of catechin in melamine-related urolithiasis via the inhibition of ROS, apoptosis, phospho-p38, and osteopontin in male Sprague-Dawley rats.FREE RADICAL RESEARCH,49(10),1249-1258.
MLA	Li, X,et al."Anti-nephrolithic potential of catechin in melamine-related urolithiasis via the inhibition of ROS, apoptosis, phospho-p38, and osteopontin in male Sprague-Dawley rats".FREE RADICAL RESEARCH 49.10(2015):1249-1258.