Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells

doi:10.3892/or.2014.3600

CORC > 兰州大学 > 兰州大学 > 第一临床医学院 > 期刊论文

	Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells
	Liu, XJ; Yang, ZX; Chen, ZF; Chen, R; Zhao, D; Zhou, YN; Qiao, L; Zhou, YN (reprint author), Lanzhou Univ, Div Gastroenterol & Hepatol, Hosp 1, 1 Gonggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
刊名	Oncology Reports
	2015-01
卷号	33 期号:1 页码:157-162
关键词	stomach carcinoma gastric cancer aerobic glycolysis LDH-A oxamate
ISSN号	1021-335X
DOI	10.3892/or.2014.3600
文献子类	Article
英文摘要	Lactate dehydrogenase A (LDH-A), which regulates glycolytic flux by catalyzing pyruvate to lactate in the cytoplasm, is believed to be one of the highly attractive therapeutic targets for cancers. Firstly, we detected the expression of LDH-A in gastric cancer (GC) cells. LDH-A inhibitor oxamate was then used to suppress the LDH-A activity in GC cells. Cell proliferation, lactic acid production, Transwell migration assay and apoptosis were assessed, respectively. The results showed that inhibition of LDH-A by oxamate decreased the lactate production. In the presence of glucose, oxamate inhibited cell proliferation in a dose-dependent manner. Flow cytometry assay further confirmed a pro-apoptotic effect of oxamate, and this was likely through increased expression of Bax, activated caspase-3, and decreased expression of Bcl-2. Therefore, we believe that oxamate inhibits cell growth, suppresses tumor invasion, and induces apoptosis in GC cells. LDH-A may be a potential therapeutic target for GC.
学科主题	Oncology
出版地	ATHENS
资助项目	中央高校基本科研业务费专项资金 ; 甘肃省技术研究与开发专项计划
项目编号	National Natural Science Funding of China [81172366] ; Fundamental Research Funds for the Central Universities [lzujbky-2012-224] ; Gansu Special Program for High Technology Research and Development [0912TCYA027]
语种	英语
WOS记录号	WOS:000346636200020
资助机构	LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125902]
专题	第一临床医学院_期刊论文
通讯作者	Zhou, YN (reprint author), Lanzhou Univ, Div Gastroenterol & Hepatol, Hosp 1, 1 Gonggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Liu, XJ,Yang, ZX,Chen, ZF,et al. Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells[J]. Oncology Reports,2015,33(1):157-162.
APA	Liu, XJ.,Yang, ZX.,Chen, ZF.,Chen, R.,Zhao, D.,...&Zhou, YN .(2015).Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells.Oncology Reports,33(1),157-162.
MLA	Liu, XJ,et al."Effects of the suppression of lactate dehydrogenase A on the growth and invasion of human gastric cancer cells".Oncology Reports 33.1(2015):157-162.