Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding

doi:10.1155/2014/834501

CORC > 兰州大学 > 兰州大学 > 第一临床医学院 > 期刊论文

	Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding
	Li, Q; Yang, WY; Qu, LL; Qi, HY; Huang, Y; Zhang, Z; Huang, Y (reprint author), Hebei Med Univ, Pharmaceut Coll, Shijiazhuang 050017, Peoples R China.
刊名	JOURNAL OF SPECTROSCOPY
	2014
页码	-
ISSN号	2314-4920
DOI	10.1155/2014/834501
文献子类	Article
英文摘要	Angelica sinensis (Oliv.) Diels combined treatment with warfarin would increase the risk of bleeding. Ferulic acid is an abundant hydroxycinnamic acid in A. sinensis and warfarin is the most widely used oral anticoagulant. The studies on supermolecular interaction of warfarin with human serum albumin (HSA) and the influence of ferulic acid on the binding would contribute to the understanding of the metabolic processes of warfarin and the effect of ferulic acid. We focus on investigating the effect of warfarin on fluorescence spectrum of human serum albumin (HSA), fluorescence quenching mechanism, binding constant, Hill coefficient, binding mode, and the effect of different ferulic acid concentrations on the binding. Warfarin quenched the intrinsic fluorescence of HSA mainly by static quenching. Accession of ferulic acid reduced the binding of HSA-warfarin. By decreasing binding constant and the Hill coefficient of warfarin with HSA, ferulic acid could improve the plasma concentration of free warfarin, which would increase the risk of bleeding. Warfarin's free concentration increased at least 50% under the condition of simulated human body. The results indicated that A. sinensis combined treatment with warfarin would increase the risk of bleeding. And the results provide an important theoretical support for warfarin used as oral anticoagulant.
学科主题	Biochemistry & Molecular Biology ; Spectroscopy
出版地	NEW YORK
资助项目	中央高校基本科研业务费专项资金 ; 甘肃省自然科学基金计划
项目编号	Natural Science Foundation of Hebei Province [08B033] ; Natural Science Foundation of Gansu Province [1010RJZA123] ; Fundamental Research Funds for the Central Universities [lzujbky-2011-102]
语种	英语
WOS记录号	WOS:000334243100001
资助机构	LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125849]
专题	第一临床医学院_期刊论文
通讯作者	Huang, Y (reprint author), Hebei Med Univ, Pharmaceut Coll, Shijiazhuang 050017, Peoples R China.
推荐引用方式 GB/T 7714	Li, Q,Yang, WY,Qu, LL,et al. Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding[J]. JOURNAL OF SPECTROSCOPY,2014:-.
APA	Li, Q.,Yang, WY.,Qu, LL.,Qi, HY.,Huang, Y.,...&Huang, Y .(2014).Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding.JOURNAL OF SPECTROSCOPY,-.
MLA	Li, Q,et al."Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding".JOURNAL OF SPECTROSCOPY (2014):-.