Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs

doi:10.1016/j.jep.2008.02.020

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	Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs
	Tang, H; Min, GT; Ge, B; Li, YM; Liu, X; Jiang, SX; Jiang, SX (reprint author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2008-05-22
卷号	117 期号:3 页码:420-426
关键词	Chi-Zhi-Huang decoction Phase I drug metabolism cocktail probe drugs high performance liquid chromatography
ISSN号	0378-8741
DOI	10.1016/j.jep.2008.02.020
文献子类	Article
英文摘要	Aim of the study: Chi-Zhi-Huang decoction (PGR) is one of the traditional Chinese medicine (TCM) preparations with unique effect on withdrawing jaundice and has been used to treat icteric patients in China for many years. In this research, we aim at to evaluate the potential activity of PGR in restoring hepatic drug metabolism in a damaged liver. Materials and methods: A cocktail approach with caffeine (10 mg/kg), dapsone (10 mg/kg) and chlorzoxazone (20 mg/kg) respectively as probe drug of cytochrome P450 (CYP) isoform of CYP 1A2, 3A4 and 2E1 was used to evaluate its possible effects on Phase I oxidative metabolism. Pretreated with three dosages of PGR water extract (0.75, 1.5 and 3 g/kg, po) for 5 days, male Wistar rats (220-240 g) were intoxicated by phenylisothiocyanate (PITC, 100 mg/kg, po) 24 h before probes intravenous injection. The pharmacokinetics of the probes in the blood was determined simultaneously by HPLC, and their non-compartmental parameters were used to evaluate the metabolic difference among the groups. Moreover, the levels of liver enzymes (ALT, AST, ALP) and bilirubins were also measured for insight of liver function. Results: The findings in this study suggest that PGR induces CYP 3A4, does not have much effect on CYP 2E1, and inhibits CYP 1A2 at high dosage. Conclusion: The current pharmacokinetic approach allowed the protective effects of PGR on oxidative drug metabolism in damaged liver to be systemically examined and will certainly help in the explanation of synergistic effect of the composites formula. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
学科主题	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
出版地	CLARE
语种	英语
WOS记录号	WOS:000256556100005
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125665]
专题	第一临床医学院_期刊论文
通讯作者	Jiang, SX (reprint author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Tang, H,Min, GT,Ge, B,et al. Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs[J]. JOURNAL OF ETHNOPHARMACOLOGY,2008,117(3):420-426.
APA	Tang, H.,Min, GT.,Ge, B.,Li, YM.,Liu, X.,...&Jiang, SX .(2008).Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs.JOURNAL OF ETHNOPHARMACOLOGY,117(3),420-426.
MLA	Tang, H,et al."Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs".JOURNAL OF ETHNOPHARMACOLOGY 117.3(2008):420-426.