A rapid reversed phase high-performance liquid chromatographic method for determination of sophoridine in rat plasma and its application to pharmacokinetics studies

doi:10.1016/j.jchromb.2006.05.023

CORC > 兰州大学 > 兰州大学 > 第一临床医学院 > 期刊论文

	A rapid reversed phase high-performance liquid chromatographic method for determination of sophoridine in rat plasma and its application to pharmacokinetics studies
	Wei, YH; Wu, XN; Liu, XF; Luo, JY; Wu, XN (reprint author), Lanzhou Univ, Dept Pharm, Hosp 1, Lanzhou 730000, Peoples R China.
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2006-10-20
卷号	843 期号:1 页码:10-14
关键词	sophoridine HPLC-DAD rat plasma
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2006.05.023
文献子类	Article
英文摘要	A high-performance liquid chromatography (HPLC) method for determining sophoridine in rat plasma was developed for application in the pharmacokinetic studies. The plasma was deproteinized with acetonitrile that contained an internal standard (ephedrine) and was separated from the aqueous layer by adding sodium chloride and sodium carbonate. The HPLC assay was carried out using a YMC-ODS column. The mobile phase was methanol-ethanol-0.01 mol l(-1) ammonium acetate buffer-triethylamine (10:0.5:89.5:0.03, v/v/v/v) (pH 6.80). The flow rate was 0.8 ml min(-1). The detection wavelength was set at 2 10 nm. The method was used to determine the concentration-time profiles of sophoridine in the plasma following oral administration or injection of sophoridine aqueous solution. The fractions of sophoridine reaching the systemic circulation were estimated for the first time by a deconvolution method. (c) 2006 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Chemistry
出版地	AMSTERDAM
语种	英语
WOS记录号	WOS:000241472400002
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125658]
专题	第一临床医学院_期刊论文
通讯作者	Wu, XN (reprint author), Lanzhou Univ, Dept Pharm, Hosp 1, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Wei, YH,Wu, XN,Liu, XF,et al. A rapid reversed phase high-performance liquid chromatographic method for determination of sophoridine in rat plasma and its application to pharmacokinetics studies[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,843(1):10-14.
APA	Wei, YH,Wu, XN,Liu, XF,Luo, JY,&Wu, XN .(2006).A rapid reversed phase high-performance liquid chromatographic method for determination of sophoridine in rat plasma and its application to pharmacokinetics studies.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,843(1),10-14.
MLA	Wei, YH,et al."A rapid reversed phase high-performance liquid chromatographic method for determination of sophoridine in rat plasma and its application to pharmacokinetics studies".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 843.1(2006):10-14.