Binding of Catalpol to Bovine Serum albumin in vitro Examined by Spectroscopy and Molecular Modeling

doi:10.1007/s10812-016-0365-3

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	Binding of Catalpol to Bovine Serum albumin in vitro Examined by Spectroscopy and Molecular Modeling
	Zhu, J; Chen, L; Hu, W; Li, J; Liu, X; Liu, X (reprint author), Henan Univ, Inst Environm & Analyt Sci, Coll Chem & Chem Engn, Kaifeng 475004, Henan, Peoples R China.; Liu, X (reprint author), Key Lab Nat Drug & Immune Engn Henan Prov, Kaifeng 475004, Henan, Peoples R China.
刊名	JOURNAL OF APPLIED SPECTROSCOPY
	2016-11
卷号	83 期号:1-5 页码:792-797
关键词	Catalpol Bovine Serum Albumin Fluorescence Uv-vis Molecular Modeling
ISSN号	0021-9037
DOI	10.1007/s10812-016-0365-3
文献子类	Article
英文摘要	This paper explores the interaction mechanisms between catalpol and bovine serum albumin (BSA) in vitro using the methods of fluorescence quenching, UV-vis absorption, synchronous fluorescence spectroscopy, and molecular modeling. The fluorescence quenching mechanism of BSA by catalpol was confirmed to be a dynamic process. In addition, the UV-vis absorption spectra of BSA in the absence and presence of catalpol provided further evidence for the quenching. Synchronous fluorescence spectra showed that the addition of catalpol did not obviously affect the microenvironment in BSA. This could be explained by the distance between catalpol and Trp residues in protein, which was deduced from the subsequent molecular docking. The theoretical results were further verified by molecular docking analysis. It showed that not only the hydrophobic force but also hydrogen bonds played a role in the interaction of catalpol with BSA.
学科主题	Spectroscopy
出版地	NEW YORK
资助项目	国家自然科学基金项目 ; 中国博士后科学基金
项目编号	National Natural Science Foundation of China [U1204304] ; China Postdoctoral Science Foundation [20110490992, 2013T60698] ; Support Plan of Science and Technology Innovation Team in Universities and Colleges in Henan Province of China [14IRTSTHN030]
语种	英语
WOS记录号	WOS:000387926000012
资助机构	NSFC ; CPSF
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/189107]
专题	药学院_期刊论文
通讯作者	Liu, X (reprint author), Henan Univ, Inst Environm & Analyt Sci, Coll Chem & Chem Engn, Kaifeng 475004, Henan, Peoples R China.; Liu, X (reprint author), Key Lab Nat Drug & Immune Engn Henan Prov, Kaifeng 475004, Henan, Peoples R China.
推荐引用方式 GB/T 7714	Zhu, J,Chen, L,Hu, W,et al. Binding of Catalpol to Bovine Serum albumin in vitro Examined by Spectroscopy and Molecular Modeling[J]. JOURNAL OF APPLIED SPECTROSCOPY,2016,83(1-5):792-797.
APA	Zhu, J.,Chen, L.,Hu, W.,Li, J.,Liu, X.,...&Liu, X .(2016).Binding of Catalpol to Bovine Serum albumin in vitro Examined by Spectroscopy and Molecular Modeling.JOURNAL OF APPLIED SPECTROSCOPY,83(1-5),792-797.
MLA	Zhu, J,et al."Binding of Catalpol to Bovine Serum albumin in vitro Examined by Spectroscopy and Molecular Modeling".JOURNAL OF APPLIED SPECTROSCOPY 83.1-5(2016):792-797.