A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata

doi:10.1016/j.jep.2016.04.016

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	A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata
	Fan, PC; Ma, HP; Hao, Y; He, XR; Sun, AJ; Jiang, W; Li, MX; Jing, LL; He, L; Ma, J
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2016-07-01
卷号	187 页码:232-238
关键词	Antifibrinolytics Hemostatics Iridoid glycosides Bleeding volume Hyperfibrinolysis
ISSN号	0378-8741
DOI	10.1016/j.jep.2016.04.016
文献子类	Article
英文摘要	Background: Fibrinolysis prevents blood clots from growing and becoming problematic. Antifibrinolytics are used as inhibitors of fibrinolysis. Aprotinin was doubted after identification of major side effects, especially on kidney. Lysine analogues has their own defects and whether they are adequate substitutes for aprotinin is still under doubt. Lamiophlomis rotata (Benth.) Kudo. was previous found to have hemostatic activity. But the active compound in L. rotata and its hemostatic mechanism were unknown. Objectives: To find the major hemostatic compound in L. rotata and identify its haemostasis mechanism. Methods: Traumatic hemorrhage model and coagulant activity assays were monitored in mice and platelets in drug treatment group and control group. Hyperfibrinolysis model was established by intravenous administration of urokinase in mice. Capillary blood clotting time (CBCT), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen and euglobulin clot lysis time (ECLT) were measured. Results: The anti-fibrinolytic activity come from 8-O-Acetyl shanzhiside methylester (ASM) one of the highest iridoid glycosides contents in TIG extracted from L rotata. ASM significantly (P < 0.05) shorten CBCT and reduced blood loss volume in vivo, but did not influence mice APTT, PT or TT. In particular, it significantly prolonged ECLT in hyperfibrinolysis mice. It indicated that ASM could inhibit fibrinolysis. ASM was also effective in CBCT, traumatic bleeding volume and ECLT in hyperfibrinolysis mice model. Conclusions: ASM was the major hemostatic compound in L rotata. The haemostasis mechanism of ASM was achieved by anti-fibrinolytic activity. ASM was a new fibrinolysis inhibitor as iridoid glycoside compound. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
学科主题	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
出版地	CLARE
资助项目	国家自然科学基金项目 ; 甘肃省杰出青年基金计划
项目编号	Chinese National Natural Science Foundation [81402848] ; Gansu Natural Science Foundation [145RJDA331]
语种	英语
WOS记录号	WOS:000378190400023
资助机构	NSFC ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179591]
专题	药学院_期刊论文
通讯作者	Ma, J; Jia, ZP (reprint author), PLA, Lanzhou Command, Gen Hosp, Dept Pharm, Lanzhou, Peoples R China.
推荐引用方式 GB/T 7714	Fan, PC,Ma, HP,Hao, Y,et al. A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata[J]. JOURNAL OF ETHNOPHARMACOLOGY,2016,187:232-238.
APA	Fan, PC.,Ma, HP.,Hao, Y.,He, XR.,Sun, AJ.,...&Jia, ZP .(2016).A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata.JOURNAL OF ETHNOPHARMACOLOGY,187,232-238.
MLA	Fan, PC,et al."A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata".JOURNAL OF ETHNOPHARMACOLOGY 187(2016):232-238.