Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometry

doi:10.1016/j.jchromb.2015.01.033

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	Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometry
	Liu, XH; Zhu, RJ; Hu, F; Guo, L; Yang, YL; Feng, SL; Feng, SL (reprint author), Lanzhou Univ, Sch Pharm, 199 Donggangxi Rd, Lanzhou 730000, Peoples R China.
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2015-04-01
卷号	986 页码:44-53
关键词	UPLC-ESI-MS CID-MS/MS MRM Flavonoids Saponins Zhenqi Fuzheng capsule Tissue distribution
ISSN号	1873-376X
DOI	10.1016/j.jchromb.2015.01.033
文献子类	Article
英文摘要	Copyright 2015 Elsevier B.V. All rights reserved.Radix Astragali (Huangqi in Chinese) and Fructus Ligustri Lucidi (Nvzhenzi in Chinese) (2:1, w/w) are combined in an herbal formulation called Zhenqi Fuzheng capsules (ZFCs) for use in China to improve immunity, promote the recovery of normal functions after surgical operations, and as the most important adjuvant therapy in cancer. In this study, the tissue distribution profiles of the six major bio-active constituents (calycosin-7-O-beta-D-glucoside, ononin, calycosin, formononetin, astragaloside IV and astragaloside II) were examined after oral administration of ZFCs to rats. All six constituents in each tissue were detected simultaneously using UPLC-ESI-MS, and the concentration of each constituent per gram of each tissue was determined. Quantification was performed using low-energy collision tandem mass spectrometry (CID-MS/MS) in multiple reaction monitoring (MRM) scan mode for the following precursor ionproduct ion transitions at m/z 447.21285.30 for calycosin-7-O-beta-D-glucoside, m/z 285.29270.38 for calycosin, m/z 431269 for ononin, m/z 269237 for formononetin, m/z 807.40627.50 for astragaloside IV, m/z 849.60669.65 for astragaloside II and m/z 633.18331.18 for the internal standard (hesperidin). The results showed that in general the tissue concentrations for all six constituents were in the following order: spleen>stomach>thymus>lung>liver>kidney>heart>testicle. The high levels in the spleen and thymus indicated that all six compounds accumulated in organs involved in the immune response, consistent with the immunity effects of ZFC. The high levels in the stomach were consistent with the oral administration of ZFC. This study was the first to compare the tissue distribution of calycosin-7-O-beta-D-glucoside with that of calycosin or of ononin with that of formononetin in rats. It was also the first study to examine the tissue distribution of astragaloside II, calycosin and formononetin following oral administration of ZFC to rats.
学科主题	Biochemistry & Molecular Biology ; Chemistry
出版地	AMSTERDAM
资助项目	国家科技支撑计划 ; 中央高校基本科研业务费专项资金
项目编号	Great Research Projects of the Ministry of Education [lzujbky-2013-198, z2012117] ; Ministry of Science and Technology of China [2011BAI05B02, 2011BAI05B00]
语种	英语
WOS记录号	WOS:000352923300008
资助机构	MOST ; MOE ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/158434]
专题	药学院_期刊论文
通讯作者	Feng, SL (reprint author), Lanzhou Univ, Sch Pharm, 199 Donggangxi Rd, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Liu, XH,Zhu, RJ,Hu, F,et al. Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,986:44-53.
APA	Liu, XH.,Zhu, RJ.,Hu, F.,Guo, L.,Yang, YL.,...&Feng, SL .(2015).Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,986,44-53.
MLA	Liu, XH,et al."Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 986(2015):44-53.