Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis

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	Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis
	Xie, XH; Wang, R; Jia, ZP; Xie, H; Zhamg, AM; Xu, J; Wang, XL; Wang, XH
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2011-11
卷号	39 期号:11 页码:1695-1700
关键词	Gastric cancer C-myc Mutation Capillary electrophoresis Single strand conformation polymorphism Restriction fragment length polymorphism 胃癌 C-myc 突变毛细管电泳单链构象多态性限制性片段长度多态性
ISSN号	0253-3820
其他题名	毛细管电泳法检测癌基因C-myc胃癌中基因点突变
通讯作者	Wang, R (reprint author), Chinese Peoples Liberat Army, Lanzhou Gen Hosp, Lanzhou 730050, Peoples R China.
中文摘要	癌基因C-myc激活和突变在胃癌形成过程中起着重要作用。通过毛细管电泳(CE)方法检测50例胃癌患者中C-myc基因突变,建立一种准确、快速诊断早期胃癌的方法。本实验采用PCR扩增胃癌及癌旁正常组织中C-myc基因第二外显子易发突变的部位基因序列,扩增样品分别经96℃变性和EcoRⅤ酶切处理,以PAGE-SSCP,CE-SSCP,CE-RFLP分别对其突变情况进行检测。优化的CE检测条件:筛分介质PEO浓度3.0%,pH 8.2,电压15 kV,温度15℃;荧光检测:λex=488 nm,λem=520 nm。检测结果:C-myc基因总突变率为20.0%(10/50)。测序分析结果显示C-my...
学科主题	Chemistry
收录类别	SCIE ; CSCD
出版地	NEW YORK
语种	中文
WOS记录号	WOS:000299462900013
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121913]
专题	药学院_期刊论文
推荐引用方式 GB/T 7714	Xie, XH,Wang, R,Jia, ZP,et al. Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(11):1695-1700.
APA	Xie, XH.,Wang, R.,Jia, ZP.,Xie, H.,Zhamg, AM.,...&Wang, XH.(2011).Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(11),1695-1700.
MLA	Xie, XH,et al."Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.11(2011):1695-1700.