Synergistic Cytotoxicity of Ampelopsin Sodium and Carboplatin in Human Non-Small Cell Lung Cancer Cell Line SPC-A1 by G(1) Cell Cycle Arrested

doi:10.1007/s11655-016-2591-1

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	Synergistic Cytotoxicity of Ampelopsin Sodium and Carboplatin in Human Non-Small Cell Lung Cancer Cell Line SPC-A1 by G(1) Cell Cycle Arrested
	Lu, L; Yang, LN; Wang, XX; Song, CL; Qin, H; Wu, YJ; Wang, XX (reprint author), Lanzhou Univ, Inst Integrat Tradit & Western Med, Sch Med, Lanzhou 730000, Peoples R China.
刊名	CHINESE JOURNAL OF INTEGRATIVE MEDICINE
	2017-02
卷号	23 期号:2 页码:125-131
关键词	ampelopsin carboplatin human non-small cell lung cancer cytotoxic effect p21
ISSN号	1672-0415
DOI	10.1007/s11655-016-2591-1
文献子类	Article
英文摘要	Objective: To evaluate the cytotoxic effects of ampelopsin sodium (Amp-Na) and carboplatin (CBP) used alone or in combination on human non-small cell lung cancer (NSCLC) cells SPC-A1 in vitro and its related mechanism. Methods: Cytotoxic effects were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The synergistic effects of the drugs were calculated with coefficient of drug interaction (CDI). Cell cycle was determined by flow cytometry (FCM). The levels of p53, p21, cyclinE, cyclinD1, and phosphorylated cyclin-dependent kinase-2 (p-CDK2) were evaluated by Western blot. Results: Amp-Na (6.25-200 mu g/mL) and CBP (3.13-100 mu g/mL) alone exhibited prominent cytotoxic activity in a concentration-dependent manner on SPC-A1 cells with 50% inhibitive concentration values of 57.07 +/- 14.46 and 34.97 +/- 6.30 mu g/mL, respectively. Drug combinations were associated with significantly higher cytotoxic effects than each drug alone (P<0.05 or 0.01). The CDI analysis confirmed the synergy of Amp-Na and CBP on inhibiting cancer cell viability across a wide concentration range (CDI <1). FCM and Western blot showed that synergistic cytotoxic effects of Amp-Na and CBP were related to G(1) arrested which mainly mediated by p21 through the inhibition of CDK2 activity independent of the p53 tumor suppressor pathway. Conclusions: Amp-Na exhibits anticancer activities and enhances the antitumor activities of CBP through up-regulation of p21 and inhibition of CDK2 activity in human NSCLC cells SPC-A1. These results suggest that Amp-Na may be applied to enhance the anticancer action of CBP.
学科主题	Integrative & Complementary Medicine
出版地	NEW YORK
资助项目	甘肃省自然科学基金计划
项目编号	Natural Science Foundation of Gansu Province, China [0710RJZA044]
语种	英语
WOS记录号	WOS:000395434900007
资助机构	GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188751]
专题	基础医学院_期刊论文
通讯作者	Wang, XX (reprint author), Lanzhou Univ, Inst Integrat Tradit & Western Med, Sch Med, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Lu, L,Yang, LN,Wang, XX,et al. Synergistic Cytotoxicity of Ampelopsin Sodium and Carboplatin in Human Non-Small Cell Lung Cancer Cell Line SPC-A1 by G(1) Cell Cycle Arrested[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2017,23(2):125-131.
APA	Lu, L.,Yang, LN.,Wang, XX.,Song, CL.,Qin, H.,...&Wang, XX .(2017).Synergistic Cytotoxicity of Ampelopsin Sodium and Carboplatin in Human Non-Small Cell Lung Cancer Cell Line SPC-A1 by G(1) Cell Cycle Arrested.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,23(2),125-131.
MLA	Lu, L,et al."Synergistic Cytotoxicity of Ampelopsin Sodium and Carboplatin in Human Non-Small Cell Lung Cancer Cell Line SPC-A1 by G(1) Cell Cycle Arrested".CHINESE JOURNAL OF INTEGRATIVE MEDICINE 23.2(2017):125-131.