Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis

doi:10.18632/oncotarget.11806

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	Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis
	Ma, H; Yao, WX; Huang, L; Jin, SH; Liu, DH; Liu, Y; Tian, X; Tian, JH; Zhou, JG; Zhou, JG (reprint author), Zunyi Med Univ, Dept Oncol, Affiliated Hosp, Zunyi 563000, Peoples R China.
刊名	ONCOTARGET
	2016-10-25
卷号	7 期号:43 页码:70128-70142
关键词	NSCLC D5F3 ALK diagnostic accuracy meta-Analysis
ISSN号	1949-2553
DOI	10.18632/oncotarget.11806
文献子类	Review
英文摘要	We conducted a pooled analysis comparing the efficacy of an immunohistochemistry (IHC) assay using the D5F3 antibody with that of fluorescence in situ hybridization (FISH) for detecting ALK gene rearrangement in NSCLC patients. A total of 32 studies involving 5805 samples were included in this review. Pooled sensitivity for D5F3 IHC was 0.97 (95% CI: 0.93-0.98), specificity was 0.99 (95% CI: 0.98-1.00), PLR was 119.20 (95% CI: 57.79-245.89), NLR was 0.03 (95% CI: 0.02-0.07), DOR was 3526.66 (95% CI: 1344.71-9249.03), and AUROC was 1.00 (95% CI: 0.99-1.00). Meta-regression revealed that specimen type was a source of heterogeneity for specificity, and specimen type and FISH signal distance were sources of heterogeneity in the joint model. Subgroup analysis revealed that sensitivity and specificity were higher when the FISH signal distance standard was >= 2 than when it was >= 1. Sensitivity was higher for tumor specimens than for cell specimens; specificity was higher for cell specimens than for tumor specimens. In conclusion, the D5F3 IHC assay was nearly as effective as FISH for detection of ALK gene rearrangement in NSCLC patients.
学科主题	Oncology ; Cell Biology
出版地	ORCHARD PARK
资助项目	国家自然科学基金项目
项目编号	NSFC (Natural Science Foundation of China) [81360351, 81660512] ; Department of Science and Technology of Guizhou Province [Qian Ke He SY [2013] 3003] ; High-level Innovative Talents Cultivation Program of Guizhou Province ; Start-Up Fund for Doctors of Zunyi Medical University ; Social Practice Program for Postgraduates of Zunyi Medical University [zy-yjs2015004] ; Applied Basic Research Programs of Science and Technology Commission Foundation of Sichuan Province [2012JY0058]
语种	英语
WOS记录号	WOS:000387448300079
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188741]
专题	基础医学院_期刊论文
通讯作者	Zhou, JG (reprint author), Zunyi Med Univ, Dept Oncol, Affiliated Hosp, Zunyi 563000, Peoples R China.
推荐引用方式 GB/T 7714	Ma, H,Yao, WX,Huang, L,et al. Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis[J]. ONCOTARGET,2016,7(43):70128-70142.
APA	Ma, H.,Yao, WX.,Huang, L.,Jin, SH.,Liu, DH.,...&Zhou, JG .(2016).Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis.ONCOTARGET,7(43),70128-70142.
MLA	Ma, H,et al."Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis".ONCOTARGET 7.43(2016):70128-70142.