Activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of ERK signaling pathway

doi:10.1016/j.peptides.2016.09.012

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	Activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of ERK signaling pathway
	Yu, HP; Zhang, N; Zhang, T; Wang, ZL; Li, N; Tang, HH; Zhang, R; Zhang, MN; Xu, BA; Fang, Q
刊名	PEPTIDES
	2016-12
卷号	86 页码:24-32
关键词	NPFF2 receptor Neurite outgrowth Neuro 2A cells MEK/ERK signaling pathway
ISSN号	0196-9781
DOI	10.1016/j.peptides.2016.09.012
文献子类	Article
英文摘要	Neurite outgrowth is an important process in neural regeneration and plasticity, especially after neural injury, and recent evidence indicates that several G(alpha 1/0) protein-coupled receptors play an important role in neurite outgrowth. The neuropeptide (NP)FF system contains two G(alpha 1/0) protein-coupled receptors, NPFF1 and NPFF2 receptors, which are mainly distributed in the central nervous system. The aim of the present study was to determine whether the NPFF system is involved in neurite outgrowth in Neuro 2A cells. We showed that Neuro 2A cells endogenously expressed NPFF2 receptor, and the NPFF2 receptor agonist dNPA inhibited cyclic adenosine monophosphate (cAMP) production stimulated by forskolin in Neuro 2A cells. We also demonstrated that NPFF and dNPA dose-dependently induced neurite outgrowth in Neuro 2A cells, which was completely abolished by the NPFF receptor antagonist RF9. Pretreatment with mitogen-activated protein kinase inhibitors PD98059 and U0126 decreased dNPA-induced neurite outgrowth. In addition, dNPA increased phosphorylation of extracellular signal-regulated kinase (ERK) in Neuro 2A cells, which was completely antagonized by pretreatment with U0126. Our results suggest that activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of the ERK signaling pathway. Moreover, NPFF2 receptor may be a potential therapeutic target for neural injury and degeneration in the future. (C) 2016 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Pharmacology & Pharmacy
出版地	NEW YORK
资助项目	国家自然科学基金项目 ; 新世纪优秀人才支持计划 ; 长江学者和创新团队发展计划
项目编号	National Natural Science Foundation of China [81273355, 81473095] ; Program for New Century Excellent Talents in University [NCET-13-0257] ; Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R27]
语种	英语
WOS记录号	WOS:000389174100004
资助机构	NSFC ; MOE
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188730]
专题	基础医学院_期刊论文
通讯作者	Fang, Q; Wang, R (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, 199 Donggang West Rd, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Yu, HP,Zhang, N,Zhang, T,et al. Activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of ERK signaling pathway[J]. PEPTIDES,2016,86:24-32.
APA	Yu, HP.,Zhang, N.,Zhang, T.,Wang, ZL.,Li, N.,...&Wang, R .(2016).Activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of ERK signaling pathway.PEPTIDES,86,24-32.
MLA	Yu, HP,et al."Activation of NPFF2 receptor stimulates neurite outgrowth in Neuro 2A cells through activation of ERK signaling pathway".PEPTIDES 86(2016):24-32.