The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil

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	The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil
	Zhang, S; Lu, ZL; Liang, HM; Fu, XM; Zhang, Y; Liu, X; Bao, GS; Jing, T; Wang, X; Wang, M
刊名	IRANIAN JOURNAL OF PUBLIC HEALTH
	2015-08
卷号	44 期号:8 页码:1061-1071
关键词	Caenorhabditis elegans 5-Fluorouracil Digital gene expression
ISSN号	2251-6085
文献子类	Article
英文摘要	Background: 5-Fluorouracil could lead to a decline in fertility in Caenorhabditis elegans. The aim of this study was to describe the mechanisms underlying such an altered fertility phenotype and to illustrate the specific genes and pathways that are involved in the related phenotypic changes in C. elegans. Methods: We isolated total RNA from the samples and used a new method called Digital Gene Expression (DGE), which can rapidly identify genes with altered transcript levels. The random genes were confirmed by real-time RTPCR. Results: We analyzed the results of two methods to draw conclusions based on a comparison between C. elegans and other harmful parasites. Compared with controls, 1147 genes were up-regulated, and 1067 were down-regulated. Overall, 101 up-regulated genes had a log2 ratio higher than 8, whereas the log2 ratio of 141 down-regulated genes was higher than 8. After mapping to the reference database, 4 pathways were confirmed to be involved in this phenomenon, with statistically significant participation from 19 genes. Conclusion: For the first time, the transcript sequence of 5-Fu-treated worms and controls was detected. We found that 4 possible pathways, i.e., ECM-receptor interaction pathway, TGF-beta signaling pathway, Focal adhesion and Hypertrophic cardiomyopathy, may be involved in the number decline in the embryos of C. elegans. Specifically, the ECM-receptor interaction pathway and Focal adhesion may be very important pathways that alter the reproduction of C. elegans.
学科主题	Public, Environmental & Occupational Health
出版地	TEHRAN
资助项目	国家自然科学基金项目 ; 中央高校基本科研业务费专项资金
项目编号	National Natural Science Funds Fund [31172174] ; Fundamental Research Funds for the Central Universities [lzujbky-2013-165]
语种	英语
WOS记录号	WOS:000360291400005
资助机构	NSFC ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179051]
专题	基础医学院_期刊论文
通讯作者	Chen, G (reprint author), Lanzhou Univ, Pathogens Biol Inst, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Zhang, S,Lu, ZL,Liang, HM,et al. The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil[J]. IRANIAN JOURNAL OF PUBLIC HEALTH,2015,44(8):1061-1071.
APA	Zhang, S.,Lu, ZL.,Liang, HM.,Fu, XM.,Zhang, Y.,...&Chen, G .(2015).The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil.IRANIAN JOURNAL OF PUBLIC HEALTH,44(8),1061-1071.
MLA	Zhang, S,et al."The Analysis of Gene Expression on Fertility Decline in Caenorhabditis elegans after the Treatment with 5-Fluorouracil".IRANIAN JOURNAL OF PUBLIC HEALTH 44.8(2015):1061-1071.