Ebb-and-Flow of Macroautophagy and Chaperone-Mediated Autophagy in Raji Cells Induced by Starvation and Arsenic Trioxide

doi:10.7314/APJCP.2014.15.14.5715

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	Ebb-and-Flow of Macroautophagy and Chaperone-Mediated Autophagy in Raji Cells Induced by Starvation and Arsenic Trioxide
	Li, CL; Wei, HL; Chen, J; Wang, B; Xie, B; Fan, LL; Li, LJ; Wei, HL (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.
刊名	ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
	2014-08-04
卷号	15 期号:14 页码:5715-5719
关键词	Chaperone-mediated autophagy (CMA) macroautophagy arsenic trioxide serum deprivation Raji cells
ISSN号	1513-7368
DOI	10.7314/APJCP.2014.15.14.5715
文献子类	Article
英文摘要	Autophagy is crucial in the maintenance of homeostasis and regenerated energy of mammalian cells. Macroautophagy and chaperone- mediated autophagy(CMA) are the two best-identified pathways. Recent research has found that in normal cells, decline of macroautophagy is appropriately parallel with activation of CMA. However, whether it is also true in cancer cells has been poorly studied. Here we focused on crosstalk and conversion between macroautophagy and CMA in cultured Burkitt lymphoma Raji cells when facing serum deprivation and exposure to a toxic compound, arsenic trioxide. The results showed that both macroautophagy and CMA were activated sequentially instead of simultaneously in starvation-induced Raji cells, and macroautophagy was quickly activated and peaked during the first hours of nutrition deprivation, and then gradually decreased to near baseline. With nutrient deprivation persisted, CMA progressively increased along with the decline of macroautophagy. On the other hand, in arsenic trioxide-treated Raji cells, macroautophagy activity was also significantly increased, but CMA activity was not rapidly enhanced until macroautophagy was inhibited by 3-methyladenine, an inhibitor. Together, we conclude that cancer cells exhibit differential responses to diverse stressor-induced damage by autophagy. The sequential switch of the first-aider macroautophagy to the homeostasis-stabilizer CMA, whether active or passive, might be conducive to the adaption of cancer cells to miscellaneous intracellular or extracellular stressors. These findings must be helpful to understand the characteristics, compensatory mechanisms and answer modes of different autophagic pathways in cancer cells, which might be very important and promising to the development of potential targeting interventions for cancer therapies via regulation of autophagic pathways.
学科主题	Oncology
出版地	GYEONGGI-DO
资助项目	长江学者和创新团队发展计划 ; 中央高校基本科研业务费专项资金
项目编号	Science and Technology Planning Project Foundation of Chengguan District, Lanzhou, Gansu Province, China [2013-7-17] ; Fundamental Research Funds for the Central Universities from Northwest University for Nationalities, China [zyz2011094] ; Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT: IRT1137]
语种	英语
WOS记录号	WOS:000343723300037
资助机构	MOE ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121732]
专题	基础医学院_期刊论文
通讯作者	Wei, HL (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Li, CL,Wei, HL,Chen, J,et al. Ebb-and-Flow of Macroautophagy and Chaperone-Mediated Autophagy in Raji Cells Induced by Starvation and Arsenic Trioxide[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(14):5715-5719.
APA	Li, CL.,Wei, HL.,Chen, J.,Wang, B.,Xie, B.,...&Wei, HL .(2014).Ebb-and-Flow of Macroautophagy and Chaperone-Mediated Autophagy in Raji Cells Induced by Starvation and Arsenic Trioxide.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(14),5715-5719.
MLA	Li, CL,et al."Ebb-and-Flow of Macroautophagy and Chaperone-Mediated Autophagy in Raji Cells Induced by Starvation and Arsenic Trioxide".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.14(2014):5715-5719.