Insulin Resistance Reduces Sensitivity to Cis-Platinum and Promotes Adhesion, Migration and Invasion in HepG2 Cells

doi:10.7314/APJCP.2014.15.7.3123

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	Insulin Resistance Reduces Sensitivity to Cis-Platinum and Promotes Adhesion, Migration and Invasion in HepG2 Cells
	Li, LJ; Li, GD; Wei, HL; Chen, J; Liu, YM; Li, F; Xie, B; Wang, B; Li, CL; Wei, HL (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
刊名	ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
	2014
卷号	15 期号:7 页码:3123-3128
关键词	Hepatocellular carcinoma insulin resistance proliferation pioglitazone hydrochloride
ISSN号	1513-7368
DOI	10.7314/APJCP.2014.15.7.3123
文献子类	Article
英文摘要	The liver is normally the major site of glucose metabolism in intact organisms and the most important target organ for the action of insulin. It has been widely accepted that insulin resistance (IR) is closely associated with postoperative recurrence of hepatocellular carcinoma (HCC). However, the relationship between IR and drug resistance in liver cancer cells is unclear. In the present study, IR was induced in HepG2 cells via incubation with a high concentration of insulin. Once the insulin-resistant cell line was established, the stability of HepG2/IR cells was further tested via incubation in insulin-free medium for another 72h. Afterwards, the biological effects of insulin resistance on adhesion, migration, invasion and sensitivity to cis-platinum (DDP) of cells were determined. The results indicated that glucose consumption was reduced in insulin-resistant cells. In addition, the expression of the insulin receptor and glucose transportor-2 was downregulated. Furthermore, HepG2/IR cells displayed markedly enhanced adhesion, migration, and invasion. Most importantly, these cells exhibited a lower sensitivity to DDP. By contrast, HepG2/IR cells exhibited decreased adhesion and invasion after treatment with the insulin sensitizer pioglitazone hydrochloride. The results suggest that IR is closely related to drug resistance as well as adhesion, migration, and invasion in HepG2 cells. These findings may help explain the clinical observation of limited efficacy for chemotherapy on a background of IR, which promotes the invasion and migration of cancer cells.
学科主题	Oncology
出版地	GYEONGGI-DO
资助项目	长江学者和创新团队发展计划 ; 中央高校基本科研业务费专项资金 ; 甘肃省青年科技基金计划
项目编号	Fundamental Research Funds for the Central Universities [lzujbky-2013-142] ; project of youth science and technology fund of Gansu province of China [1308RJYA055] ; Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT: IRT1137]
语种	英语
WOS记录号	WOS:000336834500030
资助机构	MOE ; LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121700]
专题	基础医学院_期刊论文
通讯作者	Wei, HL (reprint author), Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Li, LJ,Li, GD,Wei, HL,et al. Insulin Resistance Reduces Sensitivity to Cis-Platinum and Promotes Adhesion, Migration and Invasion in HepG2 Cells[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(7):3123-3128.
APA	Li, LJ.,Li, GD.,Wei, HL.,Chen, J.,Liu, YM.,...&Wei, HL .(2014).Insulin Resistance Reduces Sensitivity to Cis-Platinum and Promotes Adhesion, Migration and Invasion in HepG2 Cells.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(7),3123-3128.
MLA	Li, LJ,et al."Insulin Resistance Reduces Sensitivity to Cis-Platinum and Promotes Adhesion, Migration and Invasion in HepG2 Cells".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.7(2014):3123-3128.