Down-regulation of thyroid hormone receptor beta 1 gene expression in gastric cancer involves promoter methylation

doi:10.1016/j.bbrc.2014.01.012

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	Down-regulation of thyroid hormone receptor beta 1 gene expression in gastric cancer involves promoter methylation
	Ling, YQ; Shi, XQ; Wang, Y; Ling, XL; Li, Q; Shi, XQ (reprint author), Lanzhou Mil Command PLA, Lanzhou Gen Hosp, Lanzhou 730050, Gansu, Peoples R China.
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2014-02-07
卷号	444 期号:2 页码:147-152
关键词	Gastric cancer Promoter methylation Thyroid hormone receptor beta 1 Quantitative methylation-specific PCR (Q-MSP)
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2014.01.012
文献子类	Article
英文摘要	Hypermethylation has been shown in the promoter region of the thyroid hormone receptor beta 1 (TR beta 1) gene in several human tumors. However, its role in gastric cancer formation is still unclear. In the study, we analyzed mRNA expression of TR beta 1 gene using real-time quantitative PCR (qPCR). A quantitative methylation-specific PCR (Q-M5P) assay was used to determine the methylation status of the TR beta 1 gene promoter region in 46 pair-matched gastric neoplastic and adjacent non-neoplastic tissues. The results showed that TR beta 1 mRNA expression was significantly reduced in gastric cancer specimens. The methylation of promoter of TR beta 1 gene in gastric cancer tissues was significantly higher than in adjacent normal tissues. Promoter hypermethylation of the TRIM gene correlated with tumor infiltration, lymph node metastasis, and distant metastasis, but it was not associated with other clinicopathological characteristics. We treated gastric cancer cell lines MKN-45, MKN-28, SGC-7901, NCI-N87, and SNU-1 with 5-Aza-2-deoxycytidine (5-Aza-dC). The results showed the expression of TR beta 1 mRNA was increased in MKN-45, MKN-28, SGC-7901, but not increased in NCI-N87 and SNU-1. These results suggest that the TR beta 1 gene plays important roles in the development of gastric cancer partially through epigenetic mechanisms. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
出版地	SAN DIEGO
资助项目	甘肃省科技支撑计划 ; 甘肃省国际科技合作计划
项目编号	Pedestal Project of Gansu Provincial Science [090NKCA126] ; International Scientific and Technological Cooperation Project in Gansu Province [1011WCGA166] ; Medical Research Fund of PLA [CLZ 11JB05]
语种	英语
WOS记录号	WOS:000331923500007
资助机构	GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121682]
专题	基础医学院_期刊论文
通讯作者	Shi, XQ (reprint author), Lanzhou Mil Command PLA, Lanzhou Gen Hosp, Lanzhou 730050, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Ling, YQ,Shi, XQ,Wang, Y,et al. Down-regulation of thyroid hormone receptor beta 1 gene expression in gastric cancer involves promoter methylation[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,444(2):147-152.
APA	Ling, YQ,Shi, XQ,Wang, Y,Ling, XL,Li, Q,&Shi, XQ .(2014).Down-regulation of thyroid hormone receptor beta 1 gene expression in gastric cancer involves promoter methylation.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,444(2),147-152.
MLA	Ling, YQ,et al."Down-regulation of thyroid hormone receptor beta 1 gene expression in gastric cancer involves promoter methylation".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 444.2(2014):147-152.